Sheffield Jeanne S, Wendel George D, McIntire Donald D, Norgard Michael V
Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
J Infect Dis. 2007 Nov 15;196(10):1509-16. doi: 10.1086/522518. Epub 2007 Oct 31.
To examine the expression of human immunodeficiency virus type 1 (HIV-1) coreceptors (CCR5 and CXCR4) by monocytic cells within human genital ulcers.
Women with primary or secondary syphilis, herpes simplex virus type 1 (HSV-1) or HSV-2 infection, or noninfectious abrasions had a biopsy sample taken from the lesion and contralateral vulva. HIV-1 coreceptor expression on CD3(+) and CD14(+) cells was analyzed by flow cytometry. Real-time reverse-transcriptase polymerase chain reaction was used to assess levels of coreceptor mRNA expression.
Women with primary or secondary syphilis or with HSV-1 or HSV-2 infection had significantly increased numbers of CD14(+) cells expressing CCR5 within the genital ulcer. This increase was also noted in the nonulcerated tissue isolated from women with syphilis and in peripheral blood mononuclear cells from women with secondary syphilis. CCR5 mRNA expression was increased in tissue obtained from syphilis lesions.
Monocytes recruited to genital ulcer disease (GUD) sites express increased levels of CCR5. This increased expression could account, at least in part, for enhanced HIV-1 transmission in the setting of GUD.
检测人类生殖器溃疡内单核细胞中1型人类免疫缺陷病毒（HIV-1）共受体（CCR5和CXCR4）的表达情况。
患有一期或二期梅毒、1型单纯疱疹病毒（HSV-1）或2型单纯疱疹病毒（HSV-2）感染，或非感染性擦伤的女性，从病变部位及对侧外阴采集活检样本。通过流式细胞术分析CD3(+)和CD14(+)细胞上HIV-1共受体的表达情况。采用实时逆转录聚合酶链反应评估共受体mRNA表达水平。
患有一期或二期梅毒、HSV-1或HSV-2感染的女性，其生殖器溃疡内表达CCR5的CD14(+)细胞数量显著增加。在梅毒女性的非溃疡组织及二期梅毒女性的外周血单核细胞中也观察到这种增加。梅毒病变组织中CCR5 mRNA表达增加。
募集到生殖器溃疡疾病（GUD）部位的单核细胞CCR5表达水平升高。这种表达增加至少部分可以解释GUD情况下HIV-1传播增强的原因。
