Galant S P, Lundak R L, Eaton L
J Immunol. 1976 Jul;117(1):48-51.
The effects of the cholinergic stimuli carbamylcholine (carbachol) and dibutyrl cyclic guanosine monophosphate (DBCGMP) were determined on both 'early' and 'total' E rosette formation. Ficoll-Hypaque-separated lymphocytes were preincubated with either carbachol or DBCGMP over a 10(-3) M to 10(-13) M dose range. Both agents significantly enhanced 'early', but not 'total' E rosette formation. Peak enhancement above control values occurred at 10(-7) M (72%) and 10(-9) M (69%) for carbachol and 10(-5) M (70%) and 10(-7) M (70%) for DBCGMP. Kinetic studies showed a rapid onset of enhancement (2.5 min) for carbachol, whereas DBCGMP required 15 min for significant enhancement to occur. The muscurinic nature of carbachol enhancement of E rosettes was demonstrated. Atropine at 10(-7) M completely abolished the carbachol effect while showing little inhibition of the DBCGMP effect on rosette formation. These studies indicate that the cholinergic stimuli carbachl and DBCGMP significantly enhance the 'early' E rosette former in man. Human T lymphocytes appear to have functional cholinergic receptors that can be blocked by the muscurinic antagonist atropine. The role of the cyclic nucleotides and their stimulants on the immune system is incompletely understood, but it would appear that they are extremely important in the differentiation and function of the T lymphocyte. E rosette formation may be a useful model in man for studying the effects of the cyclic nucleotides on the human T lymphocyte.
研究了胆碱能刺激物氨甲酰胆碱（卡巴胆碱）和二丁酰环磷酸鸟苷（DBCGMP）对“早期”和“总”E花环形成的影响。用Ficoll-Hypaque分离的淋巴细胞在10⁻³M至10⁻¹³M的剂量范围内预先与卡巴胆碱或DBCGMP孵育。两种药物均显著增强“早期”E花环形成，但对“总”E花环形成无影响。卡巴胆碱在10⁻⁷M（72%）和10⁻⁹M（69%）时以及DBCGMP在10⁻⁵M（70%）和10⁻⁷M（70%）时出现高于对照值的峰值增强。动力学研究表明，卡巴胆碱增强作用起效迅速（2.5分钟），而DBCGMP需要15分钟才出现显著增强。证实了卡巴胆碱增强E花环形成具有毒蕈碱样性质。10⁻⁷M的阿托品完全消除了卡巴胆碱的作用，而对DBCGMP对花环形成的作用几乎没有抑制。这些研究表明，胆碱能刺激物卡巴胆碱和DBCGMP显著增强人“早期”E花环形成细胞。人T淋巴细胞似乎具有功能性胆碱能受体，可被毒蕈碱拮抗剂阿托品阻断。环核苷酸及其刺激物在免疫系统中的作用尚未完全明了，但似乎它们在T淋巴细胞的分化和功能中极其重要。E花环形成可能是研究环核苷酸对人T淋巴细胞作用的一个有用的人体模型。
