Grieco M H, Siegel I, Goel Z
J Allergy Clin Immunol. 1976 Jul;58(1 PT. 2):149-59. doi: 10.1016/0091-6749(76)90150-0.
Early rosette formation by T lymphocytes appears to be modulated by cyclic nucleotides. Dibutyryl cyclic 3',5' adenosine monophosphate (cyclic AMP) 10(-2) M inhibited E rosette formation up to 83%, while 10(-6) M 8-bromo-cyclic guanosine monophosphate (cyclic GMP) increased rosette formation maximally to 67.4% with less pronounced effect at increased concentrations. T lymphocyte receptors for beta adrenergic, alpha adrenergic, and muscarinic cholinergic agonists appear to influence rosette formation. Isoproternol 10(-2) M induced 67.3% inhibition, while phenylephrine 10(-5) M and carbamylcholine 10(-4) M were associated with enhanced rosette formation of 67.2% and 57.8%, respectively. Selective blockade with propranolol, phentolamine, and atropine indicated the presence of separate receptor sites. The lack of effect of histamine at concentrations of 10(-3) M and above suggests the absence of specific recepotrs on peripheral human T lymphocytes.
T淋巴细胞早期玫瑰花结形成似乎受环核苷酸调节。10(-2)M的二丁酰环3',5'腺苷单磷酸（环磷酸腺苷）抑制E玫瑰花结形成达83%，而10(-6)M的8-溴环鸟苷单磷酸（环磷酸鸟苷）使玫瑰花结形成最大增加至67.4%，浓度增加时作用减弱。β肾上腺素能、α肾上腺素能和毒蕈碱胆碱能激动剂的T淋巴细胞受体似乎影响玫瑰花结形成。10(-2)M的异丙肾上腺素诱导67.3%的抑制，而10(-5)M的去氧肾上腺素和10(-4)M的氨甲酰胆碱分别使玫瑰花结形成增强67.2%和57.8%。用普萘洛尔、酚妥拉明和阿托品进行选择性阻断表明存在不同的受体位点。组胺在10(-3)M及以上浓度时无作用，提示外周人T淋巴细胞不存在特异性受体。
