Dingsheng Lin, Zengbing Liu, Dong Huang
Department of Orthopaedics, Southern Medical University, Guangdong No.2 Province people's Hospital, Guangdong, China; Department of Hand Surgeries, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Department of Orthopaedics, Southern Medical University, Guangdong No.2 Province people's Hospital, Guangdong, China; Department of Hand Surgeries, The No.4 People's Hospital of Hengshui, Hengshui, China.
Iran J Basic Med Sci. 2016 Nov;19(11):1166-1170.
The aim of this study is to determine the effects of progesterone treatment on the survival of random skin flaps.
McFarlane flaps were established and 40 male rats were randomly assigned to the progesterone-treated as the test group or normal saline-treated as the control group. Progesterone or normal saline (10 mg/kg) was administered intraperitoneally once daily. On postoperative day 2, malondialdehyde (MDA) and superoxide dismutase (SOD) were detected using test kits. Flap survival rates were evaluated with transparent graph paper under direct visualization, the levels of inflammation were examined by haematoxylin and eosin (H&E) staining, and the expression of vascular endothelial growth factor (VEGF) was immunohistochemically evaluated on day 7.
Compared to that in the control group, the mean survival area was significantly larger in the progesterone group. SOD activity was increased significantly, but the MDA levels in the test group were decreased. H&E-stained slices revealed that inflammation was inhibited in the test group. VEGF expression markedly increased in the progesterone group.
This study showed that progesterone administered intraperitoneally significantly improved random skin flap survival in rats.
本研究旨在确定孕酮治疗对随意皮瓣存活的影响。
制备麦克法兰皮瓣，将40只雄性大鼠随机分为孕酮治疗组（试验组）和生理盐水治疗组（对照组）。每天腹腔注射一次孕酮或生理盐水（10mg/kg）。术后第2天，使用试剂盒检测丙二醛（MDA）和超氧化物歧化酶（SOD）。在直接观察下用透明方格纸评估皮瓣存活率，通过苏木精和伊红（H&E）染色检查炎症水平，并在第7天通过免疫组织化学评估血管内皮生长因子（VEGF）的表达。
与对照组相比，孕酮组的平均存活面积显著更大。试验组SOD活性显著增加，但MDA水平降低。H&E染色切片显示试验组炎症受到抑制。孕酮组VEGF表达明显增加。
本研究表明，腹腔注射孕酮可显著提高大鼠随意皮瓣的存活率。
