Nowak Marcin, Madej Janusz A, Dziegiel Piotr
Department of Pathological Anatomy, Pathophysiology, Microbiology and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
In Vivo. 2007 Sep-Oct;21(5):751-6.
This study aimed at immunocytochemical demonstration of E-cadherin, beta-catenin and Ki-67 expressions and the examination of correlation between these markers in primary fibrosarcomas in dogs.
Material for the study was sampled in the course of surgery from 24 mongrel dogs aged 5 to 16 years. The neoplastic tumors were subjected to histopathological verification and immunohistochemical reactions were performed to detect the studied markers. Microphotographs of the preparations were subjected to computer-assisted image analysis using the MultiScaneBase V 14.02 software.
Expression of beta-catenin was detected in all tumours examined while E-cadherin was expressed in only 8.2%. Expression of the Ki-67 proliferation-associated antigen was noted in over 33% of the tumours.
The lack of correlation (r=-0.1035) between expression of Ki-67 and that of beta-catenin detected here in contrast to the high values of Ki-67 antigen found as a prognostic factor in many other studies allowed us to conclude that the presence of beta-catenin in cells of soft tissue fibrosarcoma in dogs manifested no unequivocal relationship to augmented proliferative potential of neoplastic cells, although it did not exclude participation of the protein in the development of this neoplasia in dogs.
本研究旨在通过免疫细胞化学方法证实犬原发性纤维肉瘤中E-钙黏蛋白、β-连环蛋白和Ki-67的表达情况，并检测这些标志物之间的相关性。
研究材料取自24只年龄在5至16岁之间的杂种犬手术过程中的样本。对肿瘤组织进行组织病理学验证，并进行免疫组化反应以检测所研究的标志物。使用MultiScaneBase V 14.02软件对制备物的显微照片进行计算机辅助图像分析。
在所检查的所有肿瘤中均检测到β-连环蛋白的表达，而E-钙黏蛋白仅在8.2%的肿瘤中表达。超过33%的肿瘤中发现了Ki-67增殖相关抗原的表达。
与许多其他研究中将Ki-67抗原高表达作为预后因素不同，本研究中检测到的Ki-67与β-连环蛋白表达之间缺乏相关性（r=-0.1035），这使我们得出结论，犬软组织纤维肉瘤细胞中β-连环蛋白的存在与肿瘤细胞增殖潜能增强并无明确关系，尽管不排除该蛋白参与犬类这种肿瘤的发生发展。
