Nowak Marcin, Madej Janusz A, Dziegiel Piotr
Department of Pathological Anatomy, Pathophysiology, Microbiology and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, Poland.
In Vivo. 2009 Jan-Feb;23(1):49-53.
Minichromosome maintenance proteins (MCM), due to their involvement in DNA replication in the course of mitosis, may provide sensitive markers of cell proliferation. Localization of MCM-3 and the extent of its expression were evaluated in mammary adenocarcinomas and soft tissue fibrosarcomas in dogs. The obtained results were compared to grades of malignancy (G) of the studied tumours.
The research material was sampled in the course of surgery in 71 dogs of various breeds, aged 4 to 14 years (50 cases of mammary adenocarcinoma and 21 cases of soft tissue fibrosarcoma). The tumours were verified by histopathology and immunohistochemistry was used to evaluate MCM-3 expression. The preparations were photographed and the images were subjected to computer-assisted image analysis using MultiScanBase Ver. 14.02 software.
Nuclear expression of MCM-3 was detected in 70% adenocarcinomas and in over 71% of fibrosarcomas. Augmented expression of MCM-3 was observed in samples of tumours manifesting higher grade of malignancy. Statistical analysis demonstrated strong positive correlation (r=0.71 for fibrosarcomas, r=0.52 for adenocarcinomas; p<0.05) between MCM-3 expression and grade of malignancy in the studied tumours.
MCM-3 may provide a sensitive and useful marker of proliferative potential in various histological types of neoplastic tumours.
微小染色体维持蛋白（MCM）由于参与有丝分裂过程中的DNA复制，可能成为细胞增殖的敏感标志物。对犬乳腺腺癌和软组织纤维肉瘤中MCM-3的定位及其表达程度进行了评估。将所得结果与所研究肿瘤的恶性程度（G）进行比较。
研究材料取自71只年龄在4至14岁的不同品种犬的手术过程中（50例乳腺腺癌和21例软组织纤维肉瘤）。通过组织病理学对肿瘤进行验证，并使用免疫组织化学评估MCM-3的表达。对标本进行拍照，并使用MultiScanBase Ver. 14.02软件对图像进行计算机辅助图像分析。
在70%的腺癌和超过71%的纤维肉瘤中检测到MCM-3的核表达。在恶性程度较高的肿瘤样本中观察到MCM-3表达增强。统计分析表明，在所研究的肿瘤中，MCM-3表达与恶性程度之间存在强正相关（纤维肉瘤r=0.71，腺癌r=0.52；p<0.05）。
MCM-3可能为各种组织学类型的肿瘤性肿瘤的增殖潜能提供一个敏感且有用的标志物。
