Liu Wei, Zhao Wei-Jun, Chen Jian-Bo, Yang Ming-min
College of Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, PR China.
Anal Chim Acta. 2007 Dec 12;605(1):41-5. doi: 10.1016/j.aca.2007.10.034. Epub 2007 Oct 26.
A CPE-HPLC (UV) method was developed for the determination of Sudan (I-IV) dyes, non-ionic surfactant Triton X-100 was used to extract and preconcentrate Sudan dyes from chilli powder prior to their determination by HPLC-UV. The separation and determination of Sudan dyes was then carried out in the HPLC-UV system with isocratic elution, and the detector was set at 500 nm. The parameters and variables that affect the extraction were investigated. Under optimum conditions: 3% of Triton X-100 (W/V), 10% of Na2CO3 (W/V), heat-assisted at 70 degrees C for 30 min. Recoveries of the Sudan dyes ranged from 80.70% to 85.45% in chilli powder by CPE method, with all the relative standard deviations of less than 3%. Limit of detection (LOD) and limit of quantification (LOQ) were in the range of 2.0-4.0 and 7.0-12.0 microg kg(-1) in chilli powder, respectively.
建立了一种CPE-HPLC（紫外）法测定苏丹红（I-IV）染料，在通过HPLC-UV测定辣椒粉中的苏丹红染料之前，使用非离子表面活性剂Triton X-100对其进行萃取和预富集。然后在HPLC-UV系统中采用等度洗脱进行苏丹红染料的分离和测定，检测器设定在500nm。研究了影响萃取的参数和变量。在最佳条件下：3%的Triton X-100（W/V），10%的Na2CO3（W/V），70℃热辅助30分钟。通过CPE法测定辣椒粉中苏丹红染料的回收率在80.70%至85.45%之间，所有相对标准偏差均小于3%。辣椒粉中检测限（LOD）和定量限（LOQ）分别在2.0-4.0和7.0-12.0μg kg(-1)范围内。
