Fan Yunchang, Chen Meilan, Shentu Chao, El-Sepai Fawzi, Wang Kaixiong, Zhu Yan, Ye Mingli
Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
Anal Chim Acta. 2009 Sep 14;650(1):65-9. doi: 10.1016/j.aca.2009.03.025. Epub 2009 Mar 24.
A simple analytical method, based on the coupling of ionic liquid-based extraction with high performance liquid chromatography (HPLC), is developed for the determination of Sudan dyes (I, II, III and IV) and Para Red in chilli powder, chilli oil and food additive samples. Two ionic liquids (ILs), 1-butyl-3-methylimidazolium hexafluorophosphate ([C(4)mim][PF(6)]) and 1-octyl-3-methylimidazolium hexafluorophosphate ([C(8)mim][PF(6)]), were compared as extraction solvents; experiments indicated that the latter possesses higher recoveries for each analyte. Parameters related to extraction of Sudan dyes and Para Red were also optimized. Under the optimal conditions, good reproducibility of extraction performance was obtained, with the relative standard deviation (RSD) values ranging from 2.0% to 3.5%. The detection limits of Sudan dyes and Para Red (LOD, S/N=3) were in the range of 7.0-8.2 microg kg(-1) for chilli powder and 11.2-13.2 microg L(-1) for chilli oil and food additive. The recoveries were in the range of 76.8-109.5% for chilli powder samples and 70.7-107.8% for chilli oil and food additive samples.
建立了一种基于离子液体萃取与高效液相色谱（HPLC）联用的简单分析方法，用于测定辣椒粉、辣椒油和食品添加剂样品中的苏丹红染料（I、II、III和IV）及对位红。比较了两种离子液体（ILs），1-丁基-3-甲基咪唑六氟磷酸盐（[C(4)mim][PF(6)]）和1-辛基-3-甲基咪唑六氟磷酸盐（[C(8)mim][PF(6)]）作为萃取溶剂；实验表明，后者对每种分析物的回收率更高。还优化了与苏丹红染料和对位红萃取相关的参数。在最佳条件下，萃取性能具有良好的重现性，相对标准偏差（RSD）值在2.0%至3.5%之间。辣椒粉中苏丹红染料和对位红的检测限（LOD，S/N = 3）在7.0 - 8.2 μg kg(-1)范围内，辣椒油和食品添加剂中的检测限在11.2 - 13.2 μg L(-1)范围内。辣椒粉样品的回收率在76.8%至109.5%之间，辣椒油和食品添加剂样品的回收率在70.7%至107.8%之间。
