有丝分裂开始时细胞内激酶活性的可视化快照。
Visual snapshots of intracellular kinase activity at the onset of mitosis.
作者信息
Dai Zhaohua, Dulyaninova Natalya G, Kumar Sanjai, Bresnick Anne R, Lawrence David S
机构信息
Department of Biochemistry, The Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
出版信息
Chem Biol. 2007 Nov;14(11):1254-60. doi: 10.1016/j.chembiol.2007.10.007.
Abstract
Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior to, but not immediately after, nuclear envelope breakdown (NEB). Furthermore, kinase activity is required for the progression from prophase into metaphase. Finally, the application of selective protein kinase C (PKC) inhibitors, in combination with the caged sensor, correlates the action of the PKC beta isoform with subsequent NEB.
摘要
通过使用光激活（笼锁）传感器，可以在精确的时间控制下获取细胞内激酶活性的可视化快照，从而提供一种在细胞分裂周期的任何时间点询问酶活性的方法。强大的蛋白激酶活性恰好在核膜破裂（NEB）之前而非之后出现。此外，从前期进入中期的进程需要激酶活性。最后，将选择性蛋白激酶C（PKC）抑制剂与笼锁传感器结合使用，可将PKCβ亚型的作用与随后的NEB关联起来。
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