Banauch D, Brümmer W, Ebeling W, Metz H, Rindfrey H, Lang H, Leybold K, Rick W, Staudinger H J
Z Klin Chem Klin Biochem. 1975 Mar;13(3):101-7.
The isolation of glucose dehydrogenase from Bacillus megaterium M 1286 is outlined. Data on the specificity of the enzyme towards carbohydrates are given. A specific method for glucose determination using this enzyme was developed. Methods and results of four variants of this glucose determination are presented: End point determination in the UV range, determination with formazan as reaction product, kinetic determination in the UV range, and continuous flow analysis in the UV range (AutoAnalyzer method).
概述了从巨大芽孢杆菌M 1286中分离葡萄糖脱氢酶的过程。给出了该酶对碳水化合物特异性的数据。开发了一种使用该酶测定葡萄糖的特定方法。介绍了该葡萄糖测定方法四种变体的方法和结果:紫外范围内的终点测定、以甲臜作为反应产物的测定、紫外范围内的动力学测定以及紫外范围内的连续流动分析(自动分析仪法)。