Ohishi Hirofumi, Odoko Mamiko, Grzeskowiak Kazimierz, Hiyama Yoichi, Tsukamoto Koji, Maezaki Naoyoshi, Ishida Toshimasa, Tanaka Tetsuaki, Okabe Nobuo, Fukuyama Keiichi, Zhou Da-Yang, Nakatani Kazuhiko
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Biochem Biophys Res Commun. 2008 Feb 8;366(2):275-80. doi: 10.1016/j.bbrc.2007.10.161. Epub 2007 Nov 20.
There are many great reports of polyamine stabilization of the Z-DNA by bridge conformation between neighboring, symmetry-related Z-DNA in the packing of crystals. However, polyamine binding to the minor groove of Z-DNA and stabilizing the Z-DNA structure has been rarely reported. We proved that the synthesized polyamines bind to the minor groove of Z-DNA and stabilize the conformation under various conditions, by X-ray crystallographic study. These polyamines consist of a polyamine nano wire structure. The modes of the polyamine interaction were changed under different conditions. It is the first example that the crystals consisted of metal free structure. This finding provides a basis for clarifying B-Z transition mechanics.
在晶体堆积中，通过相邻、对称相关的Z-DNA之间的桥构象实现多胺对Z-DNA的稳定作用，已有许多出色的报道。然而，多胺与Z-DNA小沟结合并稳定Z-DNA结构的情况却鲜有报道。通过X射线晶体学研究，我们证明了合成的多胺在各种条件下都能与Z-DNA小沟结合并稳定其构象。这些多胺由多胺纳米线结构组成。在不同条件下，多胺的相互作用模式会发生变化。这是首个由无金属结构组成晶体的例子。这一发现为阐明B-Z转变机制提供了基础。
