通过微晶电子衍射法测定DNA晶体的结构。
Structure determination of a DNA crystal by MicroED.
作者信息
Haymaker Alison, Bardin Andrey A, Gonen Tamir, Martynowycz Michael W, Nannenga Brent L
机构信息
Biodesign Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, 727 East Tyler Street, Tempe, AZ 85287, USA; School for Engineering of Matter, Transport and Energy, Arizona State University, Tempe, AZ, USA.
Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Physiology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.
出版信息
Structure. 2023 Dec 7;31(12):1499-1503.e2. doi: 10.1016/j.str.2023.07.005. Epub 2023 Aug 3.
Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG) DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG) DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.
微晶电子衍射（MicroED）是一种强大的工具，可用于确定来自各种生物分子、化学和材料样品的微晶的高分辨率结构。在本研究中，我们将MicroED应用于DNA晶体，此前尚未使用该技术对其进行分析。我们利用d（CGCGCG）DNA双链体作为模型样品，并采用低温聚焦离子束研磨来制备用于衍射数据收集的薄片。MicroED数据收集及后续处理得到了d（CGCGCG）DNA分辨率为1.10 Å的结构，证明了低温聚焦离子束研磨和MicroED在核酸晶体研究中的成功应用。
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