Karadaglić Dejan
University of Liverpool, School of Biological Sciences, Biosciences Building, Crown Street, Liverpool L69 7ZB, UK.
Micron. 2008;39(3):302-10. doi: 10.1016/j.micron.2007.10.001. Epub 2007 Oct 12.
We present a theoretical analysis of the image formation in conventional brightfield reflection microscopes with optical sectioning property via structured illumination. We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. Some effects of spherical aberrations are included in the analysis. The theoretical considerations are compared with and confirmed by experimental results.
我们对具有光学切片特性的传统明场反射显微镜通过结构照明进行图像形成进行了理论分析。我们表明，用这种方法获得的光学切片图像具有与共聚焦显微镜获得的图像相当的光学切片强度。我们进一步表明，传递函数行为与真正的共聚焦仪器的行为直接可比。分析中包括了一些球差的影响。理论考量与实验结果进行了比较并得到了实验结果的证实。
