Karadaglić Dejan, Wilson Tony
University of Liverpool, School of Biological Sciences, Biosciences Building, Crown Street, Liverpool L69 7ZB, UK.
Micron. 2008 Oct;39(7):808-18. doi: 10.1016/j.micron.2008.01.017. Epub 2008 Feb 2.
We present a theoretical analysis of the image formation in structured illumination wide-field fluorescence microscopy (SIWFFM). We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. The theoretical considerations are compared with and confirmed by experimental results.
我们对结构光照宽场荧光显微镜（SIWFFM）中的图像形成进行了理论分析。我们表明，用这种方法获得的光学切片图像具有与共聚焦显微镜获得的图像相当的光学切片强度。我们进一步表明，传递函数行为与真正的共聚焦仪器直接可比。理论考量与实验结果进行了比较并得到了实验结果的证实。
