Laurent Camille, de Paiva Geisilene Rusno, Ysebaert Loic, Laurent Guy, March Michel, Delsol Georges, Brousset Pierre
INSERM, U.563, Physiopathology Center of Toulouse-Purpan, Paul-Sabatier University, and Department of Pathology, CHU Purpan, Toulouse, France.
Am J Clin Pathol. 2007 Dec;128(6):974-80. doi: 10.1309/LREBX069UXDYMBXV.
We studied the distribution of lymphoid cells in the bone marrow of 10 follicular lymphoma (FL) cases in complete response after immunochemotherapy but with nodular lymphoid infiltrates mimicking persistent lymphoma nodules. Immunohistochemical analysis showed that most of these cells displayed a T-cell phenotype with important proportions of regulatory T cells (CD3+/CD4+/FOXP3+) and mast cells. These populations were also present before treatment. Whereas no CD20+ cells were observed, immature B cells (CD79a+/terminal deoxynucleotidyl transferase+/CD20-) were detected. These cells were scarce before immunochemotherapy, suggesting that immunochemotherapy enabled their expansion. Fluorescence in situ hybridization and quantitative polymerase chain reaction failed to detect residual lymphoma cells in 5 cases with the t(14;18) translocation. Our study describes 2 important features in bone marrow in FL following immunochemotherapy. It is probable that the accumulation of regulatory T cells has some role in the control of FL. The expansion of nonmalignant B cells reflects the regeneration of B-cell lineage following immunochemotherapy.
我们研究了10例滤泡性淋巴瘤（FL）患者骨髓中淋巴细胞的分布情况，这些患者在免疫化疗后处于完全缓解状态，但骨髓中存在结节状淋巴浸润，类似持续性淋巴瘤结节。免疫组织化学分析显示，这些细胞大多表现为T细胞表型，其中调节性T细胞（CD3+/CD4+/FOXP3+）和肥大细胞占重要比例。这些细胞群在治疗前也存在。虽然未观察到CD20+细胞，但检测到了未成熟B细胞（CD79a+/末端脱氧核苷酸转移酶+/CD20-）。这些细胞在免疫化疗前很少见，提示免疫化疗促使其扩增。荧光原位杂交和定量聚合酶链反应未能在5例存在t(14;18)易位的病例中检测到残留淋巴瘤细胞。我们的研究描述了免疫化疗后FL骨髓中的两个重要特征。调节性T细胞的积累可能在FL的控制中发挥一定作用。非恶性B细胞的扩增反映了免疫化疗后B细胞谱系的再生。
