Tomita Takehiro, Meehan Brian, Wongkattiya Nalin, Malmo Jakob, Pullinger Gillian, Leigh James, Deighton Margaret
Department of Biotechnology and Environmental Biology, RMIT University, Bundoora West Campus, Bundoora, Melbourne, Victoria 3083, Australia.
Appl Environ Microbiol. 2008 Jan;74(1):114-24. doi: 10.1128/AEM.01373-07. Epub 2007 Nov 16.
Multilocus sequence typing analysis of Streptococcus uberis has identified a cluster of isolates associated with clinical and subclinical mastitis and a cluster associated with cows with low somatic cell counts in their milk. Specific groups of genotypes (global clonal complex [GCC] sequence type 5s [ST5s] and GCC ST143s) were highly associated (P = 0.006) with clinical and subclinical mastitis and may represent a lineage of virulent isolates, whereas isolates belonging to GCC ST86 were associated with low-cell-count cows. This study has, for the first time, demonstrated the occurrence of identical sequence types (ST60 and ST184) between different continents (Australasia and Europe) and different countries (Australia and New Zealand). The standardized index of association and the empirical estimation of the rate of recombination showed substantial recombination within the S. uberis population in Australia, consistent with previous multilocus sequence type analyses.
乳房链球菌的多位点序列分型分析已确定了一组与临床和亚临床乳腺炎相关的分离株，以及一组与牛奶中体细胞数低的奶牛相关的分离株。特定的基因型组（全球克隆复合体[GCC]序列类型5[ST5]和GCC ST143）与临床和亚临床乳腺炎高度相关（P = 0.006），可能代表了一种毒性分离株谱系，而属于GCC ST86的分离株与体细胞数低的奶牛相关。本研究首次证明了不同大陆（澳大拉西亚和欧洲）和不同国家（澳大利亚和新西兰）之间存在相同的序列类型（ST60和ST184）。关联标准化指数和重组率的经验估计表明，澳大利亚乳房链球菌群体内存在大量重组，这与之前的多位点序列类型分析一致。
