Zouharová Monika, Matiašovic Ján, Gebauer Jan, Matiašková Katarína, Nedbalcová Kateřina
Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, 62100 Brno, Czech Republic.
Pathogens. 2023 Nov 22;12(12):1378. doi: 10.3390/pathogens12121378.
is one of the primary causative agents of mastitis, a clinically and economically significant disease that affects dairy cattle worldwide. In this study, we analyzed 140 strains isolated from mastitis milk samples collected from 74 cow herds in the Czech Republic. We employed whole-genome sequencing to screen for the presence of antimicrobial resistance (AMR) genes and genes encoding virulence factors, and to assess their genetic relationships. Our analysis revealed the presence of 88 different sequence types (STs), with 41% of the isolates assigned to global clonal complexes (GCCs), the majority of which were affiliated with GCC5. The STs identified were distributed across the major phylogenetic branches of all currently known STs. We identified fifty-one putative virulence factor genes, and the majority of isolates carried between 27 and 29 of these genes. A tendency of virulence factors and AMR genes to cluster with specific STs was observed, although such clustering was not evident within GCCs. Principal component analysis did not reveal significant diversity among isolates when grouped by GCC or ST prevalence. The substantial genomic diversity and the wide array of virulence factors found in strains present a challenge for the implementation of effective anti-mastitis measures.
是乳腺炎的主要致病因子之一，乳腺炎是一种在临床上和经济上都很重要的疾病，影响着全球的奶牛。在本研究中，我们分析了从捷克共和国74个牛群采集的乳腺炎乳样中分离出的140株菌株。我们采用全基因组测序来筛选抗菌抗性（AMR）基因和编码毒力因子的基因，并评估它们的遗传关系。我们的分析揭示了88种不同的序列类型（STs）的存在，41%的分离株属于全球克隆复合体（GCCs），其中大多数隶属于GCC5。所鉴定的STs分布在所有目前已知STs的主要系统发育分支中。我们鉴定出51个假定的毒力因子基因，大多数分离株携带其中27至29个基因。观察到毒力因子和AMR基因有与特定STs聚类的趋势，尽管这种聚类在GCCs中并不明显。主成分分析未显示按GCC或ST流行率分组时分离株之间存在显著差异。菌株中存在的大量基因组多样性和广泛的毒力因子对实施有效的抗乳腺炎措施构成了挑战。
