Comelli Daniela, Valentini Gianluca, Cubeddu Rinaldo, Toniolo Lucia
INFM-Dipartimento di Fisica and CEQSE-CNR, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133, Milan, Italy.
Appl Spectrosc. 2005 Sep;59(9):1174-81. doi: 10.1366/0003702055012663.
We developed a combined procedure for the analysis of works of art based on a portable system for fluorescence imaging integrated with analytical measurements on microsamples. The method allows us to localize and identify organic and inorganic compounds present on the surface of artworks. The fluorescence apparatus measures the temporal and spectral features of the fluorescence emission, excited by ultraviolet (UV) laser pulses. The kinetic of the emission is studied through a fluorescence lifetime imaging system, while an optical multichannel analyzer measures the fluorescence spectra of selected points. The chemical characterization of the compounds present on the artistic surfaces is then performed by means of analytical measurements on microsamples collected with the assistance of the fluorescence maps. The previous concepts have been successfully applied to study the contaminants on the surface of Michelangelo's David. The fluorescence analysis combined with Fourier transform infrared (FT-IR) measurements revealed the presence of beeswax, which permeates most of the statue surface, and calcium oxalate deposits mainly arranged in vertical patterns and related to rain washing.
我们开发了一种基于便携式荧光成像系统与微量样品分析测量相结合的艺术品分析方法。该方法使我们能够定位和识别艺术品表面存在的有机和无机化合物。荧光装置测量由紫外（UV）激光脉冲激发的荧光发射的时间和光谱特征。通过荧光寿命成像系统研究发射动力学，而光学多通道分析仪测量选定点的荧光光谱。然后，借助荧光图收集的微量样品进行分析测量，对艺术品表面存在的化合物进行化学表征。先前的概念已成功应用于研究米开朗基罗的《大卫》表面的污染物。荧光分析与傅里叶变换红外（FT-IR）测量相结合，揭示了蜂蜡的存在，蜂蜡渗透到雕像的大部分表面，草酸钙沉积物主要呈垂直分布，与雨水冲刷有关。
