Liang Xingguo, Takenaka Nobutaka, Nishioka Hidenori, Asanuma Hiroyuki
Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
Nucleic Acids Symp Ser (Oxf). 2007(51):169-70. doi: 10.1093/nass/nrm085.
Previously, photoregulation of DNA hybridization was achieved by introducing nonsubstituted azobenzene via a D-threoninol linker: DNA duplex formed (ON) after visible light irradiation (planar trans-form), whereas the duplex dissociated (OFF) after UV light irradiation (non-planar cis-form). In this study, for more efficient photoregulation of DNA functions, the reverse switch that can turn on duplex formation with UV, and turn off it with visible light irradiation was designed. When para-isopropylazobenzene (p-(i)PrAzo) was introduced into DNA via a L-thereoninol linker, the photoswitching direction was completely reversed: the duplex involving non-planar cis-p-(i)PrAzo was much more stable than that involving planar trans-form.
此前，通过经由D-苏糖醇连接子引入未取代的偶氮苯实现了DNA杂交的光调控：可见光照射后（平面反式构象）形成DNA双链体（开），而紫外光照射后（非平面顺式构象）双链体解离（关）。在本研究中，为了更有效地对DNA功能进行光调控，设计了一种反向开关，其在紫外光照射下开启双链体形成，而在可见光照射下关闭双链体形成。当对异丙基偶氮苯（p-(i)PrAzo）经由L-苏糖醇连接子引入DNA时，光开关方向完全反转：包含非平面顺式-p-(i)PrAzo的双链体比包含平面反式构象的双链体稳定得多。
