用于RNA功能光开关的光响应性RNA的开发。

Development of photoresponsive RNA towards photoswitching of RNA functions.

作者信息

Itoh Hiroshi, Nishioka Hidenori, Liang Xingguo, Asanu Hiroyuki

机构信息

Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2007(51):171-2. doi: 10.1093/nass/nrm086.

DOI:10.1093/nass/nrm086

PMID:18029641

Abstract

By introducing azobenzene into RNA via a D-threoninol linker, the photoresponsive RNA was developed for the photoswitching of RNA hybridization. The T(m) measurements showed that RNA/RNA duplex formation and dissociation could be efficiently photoregulated. The difference in T(m) between trans- and cis-form (DeltaDeltaT(m)) was as large as 9 approximately 12 degrees C when azobenzene was tethered at the central position of a 10-nt-long RNA. Interestingly, photoregulation ability of the azobenzene introduced in RNA was even greater than that in DNA for photoswitching the corresponding duplex formation. The constructed photoresponsive RNA is promising to be applied for the photoregulation of RNA functions such as Ribozyme activity, RNAi, pre-mRNA editing, and aptamer complex formation.

摘要

通过经由D-苏糖醇接头将偶氮苯引入RNA，开发了用于RNA杂交光开关的光响应性RNA。熔点（T(m)）测量表明，RNA/RNA双链体的形成和解离可以有效地进行光调节。当偶氮苯连接在10个核苷酸长的RNA的中心位置时，反式和顺式之间的熔点差异（ΔΔT(m)）高达约9至12℃。有趣的是，引入RNA中的偶氮苯对相应双链体形成进行光开关的光调节能力甚至大于DNA中的。构建的光响应性RNA有望应用于RNA功能的光调节，如核酶活性、RNA干扰、前体mRNA编辑和适体复合物形成。