通过偶氮苯连接的DNA对核糖核酸酶H介导的RNA消化进行光调节。

Photoregulation of RNA digestion by RNase H with azobenzene-tethered DNA.

作者信息

Matsunaga Daijiro, Asanuma Hiroyuki, Komiyama Makoto

机构信息

Research Center for Advance Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

出版信息

J Am Chem Soc. 2004 Sep 22;126(37):11452-3. doi: 10.1021/ja0471976.

DOI:10.1021/ja0471976

PMID:15366887

Abstract

RNA digestion by RNase H, which is responsible for the antisense effect, was efficiently photoregulated by use of the duplex of azobenzene-tethered sense DNA and native antisense DNA. In the dark, RNA digestion was suppressed because antisense DNA was strongly hybridized with azobenzene-tethered sense DNA, and accordingly RNA was isolated. On UV irradiation, antisense DNA was released from the azobenzene-tethered DNA due to the trans-to-cis isomerization and hybridized with RNA, which was digested by RNase H.

摘要

负责反义效应的核糖核酸酶H对RNA的消化作用，可通过使用偶氮苯连接的正义DNA与天然反义DNA的双链体进行有效光调节。在黑暗中，RNA消化受到抑制，因为反义DNA与偶氮苯连接的正义DNA强烈杂交，因此RNA被分离出来。在紫外线照射下，由于反式到顺式的异构化，反义DNA从偶氮苯连接的DNA中释放出来，并与RNA杂交，然后被核糖核酸酶H消化。