Bousquet M, Saint-Pierre M, Julien C, Salem N, Cicchetti F, Calon F
Centre de Recherche en Endocrinologie Moléculaire et Oncologique, Centre Hospitalier de l'Université Laval, Québec, Canada.
FASEB J. 2008 Apr;22(4):1213-25. doi: 10.1096/fj.07-9677com. Epub 2007 Nov 21.
In this study, we examined whether omega-3 (n-3) polyunsaturated fatty acids (PUFAs) may exert neuroprotective action in Parkinson's disease, as previously shown in Alzheimer's disease. We exposed mice to either a control or a high n-3 PUFA diet from 2 to 12 months of age and then treated them with the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP; 140 mg/kg in 5 days). High n-3 PUFA dietary consumption completely prevented the MPTP-induced decrease of tyrosine hydroxylase (TH)-labeled nigral cells (P<0.01 vs. MPTP mice on control diet), Nurr1 mRNA (P<0.01 vs. MPTP mice on control diet), and dopamine transporter mRNA levels (P<0.05 vs. MPTP mice on control diet) in the substantia nigra. Although n-3 PUFA dietary treatment had no effect on striatal dopaminergic terminals, the high n-3 PUFA diet protected against the MPTP-induced decrease in dopamine (P<0.05 vs. MPTP mice on control diet) and its metabolite dihydroxyphenylacetic acid (P<0.05 vs. MPTP mice on control diet) in the striatum. Taken together, these data suggest that a high n-3 PUFA dietary intake exerts neuroprotective actions in an animal model of Parkinsonism.
在本研究中，我们检验了ω-3（n-3）多不饱和脂肪酸（PUFA）是否如先前在阿尔茨海默病中所显示的那样，在帕金森病中发挥神经保护作用。我们从2月龄至12月龄将小鼠暴露于对照饮食或高n-3 PUFA饮食中，然后用神经毒素1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP；5天内140 mg/kg）对它们进行处理。高n-3 PUFA饮食完全预防了MPTP诱导的黑质中酪氨酸羟化酶（TH）标记的黑质细胞减少（与对照饮食的MPTP小鼠相比，P<0.01）、Nurr1 mRNA减少（与对照饮食的MPTP小鼠相比，P<0.01）以及多巴胺转运体mRNA水平降低（与对照饮食的MPTP小鼠相比，P<0.05）。尽管n-3 PUFA饮食处理对纹状体多巴胺能终末没有影响，但高n-3 PUFA饮食可防止MPTP诱导的纹状体中多巴胺减少（与对照饮食的MPTP小鼠相比，P<0.05）及其代谢产物二羟基苯乙酸减少（与对照饮食的MPTP小鼠相比，P<0.05）。综上所述，这些数据表明高n-3 PUFA饮食摄入量在帕金森病动物模型中发挥神经保护作用。
