Kunihiro Tadao, Miyazaki Tomoaki, Uramoto Yuuta, Kinoshita Kyoko, Inoue Akihiro, Tamaki Sayaka, Hama Daigo, Tsutsumi Hiroaki, Ohwada Kouichi
Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Kumamoto, Japan.
Mar Pollut Bull. 2008;57(1-5):68-77. doi: 10.1016/j.marpolbul.2007.10.009. Epub 2007 Nov 26.
We monitored seasonal changes of the abundance and composition of microorganisms in the fish-farm sediment in Kusuura Bay, Amakusa, Japan, using the quinone profiling technique, during bioremediation by introducing cultured colonies of polychaete, Capitella sp. I. In November 2004, approximately 9.2 million cultured worms were transferred to the fish-farm sediment, which increased rapidly, and reached 458.5 gWW/m(2) (528,000 indiv./m(2)) in March 2005. During this fast-increasing period of Capitella, the microbial quinone content of the surface sediment (0-2 cm) also increased markedly, and reached 237 micromol/m(2) in January 2005, although the water temperature decreased to the lowest levels in the year. Particularly, the mole fraction of ubiquinone-10 in total quinones in the sediment, indicating the presence of alpha subclass of Proteobacteria, increased by 9.3%. These facts suggest that the bacterial growth was enhanced markedly by the biological activities of worms in the sediment, and the bacteria played an important role in the decomposition of the organic matter in the sediment.
我们在日本天草久住浦湾的养鱼场沉积物中，通过引入多毛纲小头虫（Capitella sp. I）的养殖群体进行生物修复期间，运用醌谱分析技术监测了微生物丰度和组成的季节性变化。2004年11月，约920万条养殖的小头虫被转移到养鱼场沉积物中，其数量迅速增加，到2005年3月达到458.5克湿重/平方米（528,000个个体/平方米）。在小头虫数量快速增长的这段时期，表层沉积物（0 - 2厘米）中的微生物醌含量也显著增加，尽管水温降至当年最低水平，但在2005年1月仍达到237微摩尔/平方米。特别是，沉积物中总醌里泛醌 - 10的摩尔分数增加了9.3%，这表明变形菌门α亚类的存在。这些事实表明，沉积物中小头虫的生物活动显著促进了细菌生长，并且细菌在沉积物中有机物的分解过程中发挥了重要作用。
