用钙溶剂对鱿鱼笔中β-几丁质的X射线衍射研究。

XRD studies of beta-chitin from squid pen with calcium solvent.

作者信息

Nagahama H, Higuchi T, Jayakumar R, Furuike T, Tamura H

机构信息

Faculty of Chemistry, Materials and Bioengineering and High Technology Research Centre, Kansai University, Suita, Osaka 564-8680, Japan.

出版信息

Int J Biol Macromol. 2008 May 1;42(4):309-13. doi: 10.1016/j.ijbiomac.2007.10.011. Epub 2007 Oct 18.

DOI:10.1016/j.ijbiomac.2007.10.011

PMID:18036656

Abstract

The crystalline structure of beta-chitin from squid pen was investigated by X-ray diffraction (XRD). The purified beta-chitin was prepared from bigfin reefsquid pen. beta-Chitin was treated with saturated calcium chloride dihydrate/alchohol (CaCl(2).2H(2)O/MeOH) solvent system at different conditions for XRD studies. The change of crystallinity of beta-chitin from squid pen was studied by using the fiber photographs on imaging plates. The results showed that the diffraction peak (010) was shifted. It means that the lattice plane (010) interplanarilly spreaded to 3.4A, when the squid pen was washed with water after treatment of Ca solvent. Furthermore, when the squid pen was dried after treatment of Ca solvent and washing with water, interplanar spacing of (010) inversely shrank to 1.1A. These results suggested that Ca solvent especially influences the plane (010) of beta-chitin structure.

摘要

通过X射线衍射（XRD）研究了鱿鱼笔中β-几丁质的晶体结构。纯化的β-几丁质由大鳍鱿鱼笔制备。β-几丁质用饱和二水合氯化钙/乙醇（CaCl₂·2H₂O/MeOH）溶剂体系在不同条件下处理以进行XRD研究。利用成像板上的纤维照片研究了鱿鱼笔中β-几丁质结晶度的变化。结果表明衍射峰（010）发生了位移。这意味着在用Ca溶剂处理后用水洗涤鱿鱼笔时，晶格平面（010）在平面间扩展到了3.4埃。此外，在用Ca溶剂处理并用水洗涤后将鱿鱼笔干燥时，（010）的平面间距反向收缩至1.1埃。这些结果表明Ca溶剂尤其会影响β-几丁质结构的（010）平面。