Yu Hao, Li Jingfen, Li Li, Song Yuefen, Yang Xiuqin, Ding Linlin, Liu Di
Animal Science College, Northeast Agricultural University, Harbin 150030, China.
J Genet Genomics. 2007 Nov;34(11):994-1000. doi: 10.1016/S1673-8527(07)60112-X.
Pig GATA-3 cDNA was obtained by reverse transcription polymerase chain reaction (RT-PCR), using in silico cloning strategy based on pig dbEST. The length of pig GATA-3 cDNA is 1,760 bp containing a 1,335 bp open reading frame (ORF), which encodes a 444 amino acid protein. Semi-quantitative RT-PCR analysis of GATA-3 mRNA expression was done using the total RNAs from different normal tissues of a large white pig. The GATA-binding family of transcription factors comprised of a subgroup of DNA-binding proteins that both bound the consensus GATA motif and contained the class IV zinc finger motif. The molecular evolution tree was constructed based on the GATA-3 amino acid sequence and class IV zinc finger motif using mega 3.1. Phylogeny analysis of GATA factors isolated from vertebrates suggested that the six distinct vertebrate GATAs had descended from a common ancestral sequence, and the topology also suggested multiple modes of evolution including gene duplication and class IV zinc finger motif recombination. These data helped the authors in illuminating the pathways of divergent and convergent evolution of the GATA-binding family of transcription factors.
基于猪dbEST的电子克隆策略，通过逆转录聚合酶链反应（RT-PCR）获得猪GATA-3 cDNA。猪GATA-3 cDNA长度为1760 bp，包含一个1335 bp的开放阅读框（ORF），编码一个444个氨基酸的蛋白质。使用大白猪不同正常组织的总RNA进行GATA-3 mRNA表达的半定量RT-PCR分析。GATA结合转录因子家族由一组DNA结合蛋白组成，这些蛋白既结合共有GATA基序，又包含IV类锌指基序。基于GATA-3氨基酸序列和IV类锌指基序，使用mega 3.1构建分子进化树。对从脊椎动物中分离出的GATA因子的系统发育分析表明，六种不同的脊椎动物GATA因子均源自一个共同的祖先序列，并且拓扑结构还表明存在多种进化模式，包括基因复制和IV类锌指基序重组。这些数据有助于作者阐明GATA结合转录因子家族的发散和趋同进化途径。
