Jin D, Tan H J, Lei T, Gan L, Chen X D, Long Q Q, Feng B, Yang Z Q
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Comp Biochem Physiol B Biochem Mol Biol. 2009 Aug;153(4):348-58. doi: 10.1016/j.cbpb.2009.04.004. Epub 2009 Apr 21.
The sirtuin family of class III histone deacetylases (HDACs) is named after their homology to yeast silent information regulator 2 (SIR2). SIR2 and its mammalian derivatives (SIRT1-7) play a central role in gene silencing, cell cycle, aging and metabolism. Here we reported cDNA cloning, chromosome mapping,expression and evolutional analysis of sirtuin genes in Sus scrofa (Tongcheng pig). Sequence analysis showed that porcine sirtuin genes contain 7 members designated SIRT1-7. Tissue distribution analysis indicated porcine sirtuin genes ubiquitously expressed but with the highest abundance in brain, spinal cord and genital tissue. In silico and radiation hybrid mapping analysis mapped porcine SIRT1-7 to the chromosomes 14q23,6q11-12, 2q29, 14q19, 7p12, 2q11, and 12p15, respectively. We also isolated and characterized genomic sequence of porcine SIRT1, which spaned a region of 31,834 bp comprising 9 exons ranging in size from 80 bpto 2121 bp. The 5' flanking genomic region preceding an open reading frame of SIRT1 has a TATA box, a small300 bp CpG island and several putative Sp1 and p53 transcription factor binding sites. Moreover, we isolated two novel splicing SIRT6 variants with 346 bp (variant 2) in-frame deletions from lung and 327 bp(variant 3) in-frame deletions from spleen and brain. This is the first systematic report of molecular cloning and characterization of sirtuin genes in pigs, which will be helpful for a better understanding of the physiological role of sirtuin proteins in pigs.
III类组蛋白去乙酰化酶（HDAC）的沉默调节蛋白家族因其与酵母沉默信息调节因子2（SIR2）的同源性而得名。SIR2及其哺乳动物衍生物（SIRT1 - 7）在基因沉默、细胞周期、衰老和代谢中发挥核心作用。在此，我们报道了猪（通城猪）沉默调节蛋白基因的cDNA克隆、染色体定位、表达及进化分析。序列分析表明，猪沉默调节蛋白基因包含7个成员，命名为SIRT1 - 7。组织分布分析显示，猪沉默调节蛋白基因在各组织中均有表达，但在脑、脊髓和生殖组织中表达量最高。通过电子克隆和辐射杂种定位分析，将猪SIRT1 - 7分别定位到染色体14q23、6q11 - 12、2q29、14q19、7p12、2q11和12p15上。我们还分离并鉴定了猪SIRT1的基因组序列，其跨度为31,834 bp，包含9个外显子，大小从80 bp到2121 bp不等。SIRT1开放阅读框之前的5'侧翼基因组区域有一个TATA盒、一个约300 bp的小CpG岛以及几个假定的Sp1和p53转录因子结合位点。此外，我们从肺中分离出两个新的剪接SIRT6变体，有346 bp（变体2）的框内缺失，从脾脏和脑中分离出有327 bp（变体3）的框内缺失。这是关于猪沉默调节蛋白基因分子克隆和特征的首次系统报道，将有助于更好地理解沉默调节蛋白在猪中的生理作用。
