Huazhong Agricultural University, Wuhan, China.
J Genet Genomics. 2009 Dec;36(12):695-702. doi: 10.1016/S1673-8527(08)60162-9.
Messenger RNA-like non-coding RNAs (mlncRNAs) are a newly identified group of non-coding RNAs (ncRNAs) that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs were obtained by computational prediction and screening, among which 72 were mapped to the porcine genome. Further analysis of 8 representative candidates revealed that these mlncRNA candidates are not highly conserved among species. Remarkably, one of the candidates, sTF35495, was found to be precursor of a putative porcine microRNA. By RACE PCR, we determined that the full length of sTF35495 was 3 kb. The protein-coding potential of this RNA was tested in silico with no significant finding. Semi-quantitative RT-PCR analysis of the subgroup of 8 candidates revealed two distinct expression profiles and two molecules were further validated by real-time PCR. The predicted pre-microRNA sequence in this study provides a potentially interesting insight into the in vivo function of porcine mlncRNAs and our findings suggest that they play key biological roles in Sus scrofa.
信使 RNA 样非编码 RNA（mlncRNAs）是一类新发现的非编码 RNA（ncRNAs），可能参与许多关键的细胞事件。在这项研究中，通过计算预测和筛选获得了 93 个候选猪 mlncRNAs，其中 72 个被映射到猪基因组上。对 8 个代表性候选物的进一步分析表明，这些 mlncRNA 候选物在物种间的保守性不高。值得注意的是，其中一个候选物 sTF35495 被发现是一个假定的猪 microRNA 的前体。通过 RACE-PCR，我们确定 sTF35495 的全长为 3kb。该 RNA 的编码蛋白潜力通过计算机进行了无显著发现的测试。对 8 个候选物的亚组进行半定量 RT-PCR 分析，揭示了两种不同的表达谱，其中两个分子通过实时 PCR 进一步验证。本研究中预测的前 microRNA 序列为猪 mlncRNAs 的体内功能提供了一个潜在的有趣见解，我们的研究结果表明，它们在 Sus scrofa 中发挥着关键的生物学作用。
