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葡萄牙城乡居民血清中赭曲霉毒素A的水平及暴露程度评估。

Levels of ochratoxin A in serum from urban and rural Portuguese populations and estimation of exposure degree.

作者信息

Lino C M, Baeta M L, Henri M, Dinis A M P, Pena A S, Silveira M I N

机构信息

Group of Bromatology CEF, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.

出版信息

Food Chem Toxicol. 2008 Mar;46(3):879-85. doi: 10.1016/j.fct.2007.10.012. Epub 2007 Oct 24.

Abstract

Urban and rural population exposure to ochratoxin A (OTA) in central zone of Portugal was investigated in three places: Coimbra, Verride and Ereira. The analytical method proposed for the determination of ochratoxin A involved extraction with chloroform-orthophosphoric acid, cleanup through an immunoaffinity column (IAC), high performance liquid chromatography (HPLC) with spectrofluorimetric detection (FD) for separation and identification of ochratoxin A, and confirmation with HPLC-FD after OTA methylation in serum. The limit of quantification of the proposed method was 0.1 microg/L for serum and 0.05 microg/L for blood. OTA recoveries in serum ranged from 70.3% to 115.3% for levels at 0.25 microg/L and 0.5 microg/L, respectively, with a within-day RSD between 8.0% and 16.2%. Ochratoxin A serum levels were evaluated in an hundred and four donors from Coimbra city, Verride, and Ereira. The study revealed a frequency of detection of 100%. The ratio of ochratoxin A level in serum to whole blood was 2.0+/-0.7. The overall concentrations range from 0.25 to 2.49 microg/L, 0.14 to 1.91 microg/L, and 0.19 to 0.96 microg/L, for samples of Verride, Ereira, and Coimbra, respectively. The mean concentration and standard deviation were 0.78+/-0.53 microg/L, 0.44+/-0.31 microg/L, and 0.42+/-0.18 microg/L for the same samples. A significant difference was found in Verride population (P-value=0.000). Levels of OTA are clearly higher in males from rural areas than in females. For all samples, a significant difference was found in Verride male population (P-value=0.014).

摘要

在葡萄牙中部地区的三个地方(科英布拉、韦里德和埃雷拉),对城乡居民接触赭曲霉毒素A(OTA)的情况进行了调查。所提出的用于测定赭曲霉毒素A的分析方法包括用氯仿 - 正磷酸进行萃取,通过免疫亲和柱(IAC)进行净化,采用配有荧光检测(FD)的高效液相色谱(HPLC)对赭曲霉毒素A进行分离和鉴定,并在血清中OTA甲基化后用HPLC - FD进行确证。所提方法的定量限血清为0.1微克/升,血液为0.05微克/升。血清中OTA在0.25微克/升和0.5微克/升水平时的回收率分别为70.3%至115.3%,日内相对标准偏差在8.0%至16.2%之间。对来自科英布拉市、韦里德和埃雷拉的104名捐赠者的血清赭曲霉毒素A水平进行了评估。研究显示检出频率为100%。血清中赭曲霉毒素A水平与全血中该毒素水平的比值为2.0±0.7。韦里德、埃雷拉和科英布拉样本的总体浓度范围分别为0.25至2.49微克/升、0.14至1.91微克/升和0.19至0.96微克/升。相同样本的平均浓度和标准偏差分别为0.78±0.53微克/升、0.44±0.31微克/升和0.42±0.18微克/升。在韦里德人群中发现有显著差异(P值 = 0.000)。农村地区男性的OTA水平明显高于女性。对于所有样本,在韦里德男性人群中发现有显著差异(P值 = 0.014)。

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