Reproductive Medical Center, Nanjing Jinling Hospital, Nanjing University, School of Medicine, Nanjing, 210002, Jiangsu, China.
Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.
Mycotoxin Res. 2020 Aug;36(3):269-276. doi: 10.1007/s12550-020-00387-8. Epub 2020 Feb 3.
This study was conducted to investigate the exposure to ochratoxin A (OTA) of populations living in Nanjing, China. Plasma samples were collected from 147 healthy adults (age 18-63 years) and analyzed for OTA by a reliable and sensitive ultra-high-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method (limit of detection 0.04 ng/mL). After enzymatic hydrolysis by β-glucuronidase/arylsulfatase, OTA was detected in 80.9% plasma samples with mean concentration of 0.26 ± 0.59 ng/mL (range 0.04-6.59 ng/mL). The estimated daily intakes of OTA based on plasma concentrations (mean 0.51 ng/kg bw/day, max 12.99 ng/kg bw/day) were lower than the tolerable daily intake published by European Food Safety Authority (EFSA) or Joint FAO/WHO Expert Committee on Food Additives (JFECFA) indicative of rare risks related to the OTA exposure in the investigated area. This study provides a valuable insight on human exposure to OTA in China. Further studies in children and elder people and in adult cohorts from other regions are recommended.
本研究旨在调查中国南京居民接触赭曲霉毒素 A（OTA）的情况。采集了 147 名健康成年人（年龄 18-63 岁）的血浆样本，并用可靠、灵敏的超高效液相色谱串联质谱法（UPLC-MS/MS）进行分析（检测限为 0.04 ng/mL）。经β-葡萄糖醛酸酶/芳基硫酸酯酶酶解后，80.9%的血浆样本中检测到 OTA，平均浓度为 0.26±0.59ng/mL（范围 0.04-6.59ng/mL）。基于血浆浓度估算的 OTA 每日摄入量（平均值为 0.51ng/kg bw/天，最大值为 12.99ng/kg bw/天）低于欧洲食品安全局（EFSA）或粮农组织/世界卫生组织食品添加剂联合专家委员会（JECFA）公布的耐受日摄入量，表明在所调查地区，与 OTA 暴露相关的风险较低。本研究为了解中国人群 OTA 暴露情况提供了有价值的信息。建议进一步在儿童、老年人和其他地区的成年人群体中开展研究。
