De Blas Bravo Isabel, Sanz Castro Ramón, López Riquelme Natividad, Tormo Díaz Consuelo, Apraiz Goyenaga David
Applus+LABAQUA (Alicante), Polígono Industrial de Las Atalayas, Calle del Dracma 16-18, 03114 Alicante, Spain.
J Trace Elem Med Biol. 2007;21 Suppl 1:14-7. doi: 10.1016/j.jtemb.2007.09.017. Epub 2007 Nov 7.
The ICP-MS for simultaneous trace element determination in human blood has prevailed as the most suitable methodology for clinical aims because of its rapidity, detection limits and minimal sample quantity needed for analysis. As the proteic matrix is high, it is necessary to fine-tune the ICP-MS Agilent 7500i with autosampler CETAC ASX-500 and ISIS System connected, and further we have to the sample pre-treatment in order to obtain good results. The study of the results shows that the best pre-treatment for blood serum samples consists of a basic treatment by 1/10 dilution with a solution of EDTA and NH(4)OH, with a detection limit of the order of mug/L and a reduction of the necessary patient sample volume to 2 mL.
用于同时测定人体血液中微量元素的电感耦合等离子体质谱仪（ICP-MS），因其分析速度快、检测限低以及所需分析样本量极少，已成为临床目标中最合适的方法。由于蛋白质基质含量高，有必要对配备CETAC ASX-500自动进样器和ISIS系统的安捷伦7500i型ICP-MS进行微调，此外，为了获得良好结果，还必须进行样品预处理。结果研究表明，血清样品的最佳预处理方法是用EDTA和NH(4)OH溶液按1/10稀释进行基本处理，检测限为微克/升量级，且可将所需患者样本体积减少至2毫升。
