Bhatia Sujata K, Kurian Joseph V
Biochemical Sciences & Engineering, Central Research and Development, DuPont Experimental Station, Wilmington, DE 19880, USA.
Biotechnol Lett. 2008 Apr;30(4):619-23. doi: 10.1007/s10529-007-9607-z. Epub 2007 Nov 27.
The Sorona family of polymers, based on corn-derived 1,3-propanediol, have recently been developed as novel bio-based materials for use in plastics, films, and fibers. In the present study, Sorona polymers were investigated for their effects on cell survival and inflammatory cell activation using in vitro mouse cell cultures. Cytotoxicity of Sorona polymers was evaluated by placing material samples in direct contact with 3T3 fibroblast cells. Both Sorona plastic and Sorona films were non-cytotoxic to fibroblasts. The inflammatory potential of Sorona samples was evaluated by exposing J774 macrophage cells to material samples, and measuring TNF-alpha release from macrophages. Sorona plastic and Sorona films did not elicit inflammatory TNF-alpha release from macrophages. These results indicate that Sorona polymers are non-cytotoxic and non-inflammatory. While the 1,3-propanediol component of Sorona 3GT is manufactured in a bacterial fermentation process, the absence of an inflammatory response to Sorona film and Sorona plastic is highly encouraging. The results are significant for the design of materials that utilize bio-based polymers.
基于玉米衍生的1,3 - 丙二醇的索罗纳聚合物家族，最近已被开发为用于塑料、薄膜和纤维的新型生物基材料。在本研究中，使用体外小鼠细胞培养来研究索罗纳聚合物对细胞存活和炎性细胞活化的影响。通过将材料样品与3T3成纤维细胞直接接触来评估索罗纳聚合物的细胞毒性。索罗纳塑料和索罗纳薄膜对成纤维细胞均无细胞毒性。通过将J774巨噬细胞暴露于材料样品并测量巨噬细胞释放的肿瘤坏死因子 - α（TNF - α）来评估索罗纳样品的炎性潜力。索罗纳塑料和索罗纳薄膜不会引发巨噬细胞释放炎性TNF - α。这些结果表明索罗纳聚合物无细胞毒性且无炎症反应。虽然索罗纳3GT的1,3 - 丙二醇成分是在细菌发酵过程中制造的，但对索罗纳薄膜和索罗纳塑料没有炎症反应是非常令人鼓舞的。这些结果对于利用生物基聚合物的材料设计具有重要意义。
