Bhatia Sujata K, Arthur Samuel D, Chenault H Keith, Kodokian George K
Biochemical Sciences and Engineering, Central Research and Development, DuPont Experimental Station, Wilmington, DE, 19880, USA.
Biotechnol Lett. 2007 Nov;29(11):1645-9. doi: 10.1007/s10529-007-9465-8. Epub 2007 Jul 17.
The effects of polysaccharide-based tissue adhesives on cell survival and inflammatory cell activation were determined using in vitro mouse cell cultures. Cytotoxicity of tissue adhesives was evaluated by placing adhesives in direct contact with 3T3 fibroblast cells. Polysaccharide-based tissue adhesives composed of dextran aldehyde and star PEG amine were non-cytotoxic to fibroblasts; in contrast, a commercial adhesive composed of 2-octyl cyanoacrylate was highly cytotoxic to fibroblasts. The inflammatory potential of tissue adhesives was evaluated by exposing J774 macrophage cells to adhesives, and measuring TNF-alpha release from macrophages. Polysaccharide-based tissue adhesives did not elicit inflammatory TNF-alpha release from macrophages. These results suggest that polysaccharide-based tissue adhesives are non-cytotoxic and non-inflammatory; the results are therefore significant in the design of in vitro cell culture systems to study biomaterials.
使用体外小鼠细胞培养来确定基于多糖的组织粘合剂对细胞存活和炎性细胞活化的影响。通过将粘合剂与3T3成纤维细胞直接接触来评估组织粘合剂的细胞毒性。由葡聚糖醛和星状聚乙二醇胺组成的基于多糖的组织粘合剂对成纤维细胞无细胞毒性；相比之下，由2-氰基丙烯酸辛酯组成的商业粘合剂对成纤维细胞具有高度细胞毒性。通过将J774巨噬细胞暴露于粘合剂并测量巨噬细胞释放的肿瘤坏死因子-α（TNF-α）来评估组织粘合剂的炎症潜力。基于多糖的组织粘合剂不会引起巨噬细胞释放炎性TNF-α。这些结果表明基于多糖的组织粘合剂无细胞毒性且无炎症；因此，这些结果对于研究生物材料的体外细胞培养系统设计具有重要意义。
