Ide Tomihiko, Komoto Satoshi, Higo-Moriguchi Kyoko, Htun Khaing Win, Myint Yi Yi, Myat Theingi Win, Thant Kyaw Zin, Thu Hlaing Myat, Win Mo Mo, Oo Htun Naing, Htut Than, Wakuda Mitsutaka, Dennis Francis Ekow, Haga Kei, Fujii Yoshiki, Katayama Kazuhiko, Rahman Shofiqur, Nguyen Sa Van, Umeda Kouji, Oguma Keiji, Tsuji Takao, Taniguchi Koki
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Nay Pyi Taw General Hospital (Central Myanmar), Nay Pyi Taw, Myanmar.
PLoS One. 2015 May 4;10(5):e0124965. doi: 10.1371/journal.pone.0124965. eCollection 2015.
G12 rotaviruses are emerging rotavirus strains causing severe diarrhea in infants and young children worldwide. However, the whole genomes of only a few G12 strains have been fully sequenced and analyzed. In this study, we sequenced and characterized the complete genomes of six G12 strains (RVA/Human-tc/MMR/A14/2011/G12P[8], RVA/Human-tc/MMR/A23/2011/G12P[6], RVA/Human-tc/MMR/A25/2011/G12P[8], RVA/Human-tc/MMR/P02/2011/G12P[8], RVA/Human-tc/MMR/P39/2011/G12P[8], and RVA/Human-tc/MMR/P43/2011/G12P[8]) detected in six stool samples from children with acute gastroenteritis in Myanmar. On whole genomic analysis, all six Myanmarese G12 strains were found to have a Wa-like genetic backbone: G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for strains A14, A25, P02, P39, and P43, and G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 for strain A23. Phylogenetic analysis showed that most genes of the six strains examined in this study were genetically related to globally circulating human G1, G3, G9, and G12 strains. Of note is that the NSP4 gene of strain A23 exhibited the closest relationship with the cognate genes of human-like bovine strains as well as human strains, suggesting the occurrence of reassortment between human and bovine strains. Furthermore, strains A14, A25, P02, P39, and P43 were very closely related to one another in all the 11 gene segments, indicating derivation of the five strains from a common origin. On the other hand, strain A23 consistently formed distinct clusters as to all the 11 gene segments, indicating a distinct origin of strain A23 from that of strains A14, A25, P02, P39, and P43. To our knowledge, this is the first report on whole genome-based characterization of G12 strains that have emerged in Myanmar. Our observations will provide important insights into the evolutionary dynamics of spreading G12 rotaviruses in Asia.
G12轮状病毒是一种正在出现的轮状病毒株，在全球范围内导致婴幼儿严重腹泻。然而，仅有少数G12毒株的全基因组被完全测序和分析。在本研究中，我们对从缅甸急性胃肠炎患儿的6份粪便样本中检测到的6株G12毒株（RVA/Human-tc/MMR/A14/2011/G12P[8]、RVA/Human-tc/MMR/A23/2011/G12P[6]、RVA/Human-tc/MMR/A25/2011/G12P[8]、RVA/Human-tc/MMR/P02/2011/G12P[8]、RVA/Human-tc/MMR/P39/2011/G12P[8]和RVA/Human-tc/MMR/P43/2011/G12P[8]）进行了测序和特征分析。通过全基因组分析，发现所有6株缅甸G12毒株均具有类似Wa株的基因骨架：A14、A25、P02、P39和P43毒株为G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1，A23毒株为G12-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1。系统发育分析表明，本研究中检测的6株毒株的大多数基因与全球流行的人G1、G3、G9和G12毒株在遗传上相关。值得注意的是，A23毒株的NSP4基因与类人牛毒株以及人毒株的同源基因关系最为密切，这表明人毒株和牛毒株之间发生了重配。此外，A14、A25、P02、P39和P43毒株在所有11个基因片段上彼此密切相关，表明这5株毒株起源于共同祖先。另一方面，A23毒株在所有11个基因片段上始终形成独特的聚类，表明A23毒株与A14、A25、P02、P39和P43毒株起源不同。据我们所知，这是关于缅甸出现的G12毒株基于全基因组特征分析的首次报告。我们的观察结果将为亚洲传播的G12轮状病毒的进化动态提供重要见解。
