Chuanchuen Rungtip, Khemtong Sirintip, Padungtod Pawin
Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2007 Sep;38(5):855-62.
In this study, a total of 122 Salmonella enterica isolates from poultry and swine were assessed for susceptibility to clinically important antibiotics and to benzalkonium chloride (BKC). All isolates were examined for the presence of the antiseptic resistance genes qacE and qacEDelta1 and intl1 (class 1 integrase). The intl1-positive strains were further investigated for the presence of the 3' conserved region. The results demonstrated widespread distribution of qacEDelta1 (27%) but no isolate with qacE was observed. The intl1 gene was identified in 23 isolates (70%) with qacEDelta1. All of the intl1-postive strains carried qacEDelta1 in 3' conserved segment, confirming that the qacEDelta1 gene is linked to the integrons. Increased MIC value to BKC was independent of the presence of qacEDelta1, and multiple antibiotic-resistant bacteria were no more tolerant to BKC than the non-multidrugresistant strains, regardless of the presence of qacEDelta1.
在本研究中，对从家禽和猪身上分离出的总共122株肠炎沙门氏菌进行了评估，检测其对临床重要抗生素和苯扎氯铵（BKC）的敏感性。检查所有分离株是否存在抗防腐剂抗性基因qacE和qacEΔ1以及intl1（1类整合酶）。对intl1阳性菌株进一步检测其3'保守区的存在情况。结果表明qacEΔ1广泛分布（27%），但未观察到携带qacE的分离株。在23株（70%）携带qacEΔ1的分离株中鉴定出intl1基因。所有intl1阳性菌株在3'保守区段都携带qacEΔ1，证实qacEΔ1基因与整合子相关。对BKC的最低抑菌浓度（MIC）值升高与qacEΔ1的存在无关，并且无论是否存在qacEΔ1，多重耐药菌对BKC的耐受性并不比非多重耐药菌株更强。
