Lynne Aaron M, Dorsey Lindsay L, David Donna E, Foley Steven L
National Farm Medicine Center, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA.
Int J Antimicrob Agents. 2009 Aug;34(2):169-72. doi: 10.1016/j.ijantimicag.2009.02.018. Epub 2009 Apr 7.
Salmonella enterica serovars Dublin, Choleraesuis and Pullorum are host-adapted serovars that cause disease primarily in cattle, swine and poultry, respectively. In addition, serovars Dublin and Choleraesuis are important human pathogens that are disproportionately associated with severe invasive infections that require antimicrobial therapy. Because of the potential increased emergence and spread of antimicrobial resistance, isolates of 42 S. enterica serovars Dublin, Choleraesuis and Pullorum were characterised to evaluate resistance. Antimicrobial susceptibility testing, detection of resistance genes and integrons, pulsed-field gel electrophoresis and plasmid analysis were carried out to characterise the isolates. Seventy-nine percent of the isolates were resistant to at least one of the antimicrobial agents tested, whilst 38% of the isolates were resistant to six or more antimicrobial agents. Resistance was most commonly detected to tetracycline (64%), streptomycin (57%) and kanamycin (52%). Overall, when resistance was seen, a corresponding resistance gene was detected 86.7% of the time. The results of this study indicate that antimicrobial resistance is a major concern in serovars Dublin and Choleraesuis isolates owing to the presence of multidrug resistance.
肠炎沙门氏菌都柏林血清型、猪霍乱血清型和鸡白痢血清型是宿主适应性血清型，分别主要在牛、猪和家禽中引起疾病。此外，都柏林血清型和猪霍乱血清型是重要的人类病原体，与需要抗菌治疗的严重侵袭性感染不成比例地相关。由于抗菌药物耐药性可能增加的出现和传播，对42株肠炎沙门氏菌都柏林血清型、猪霍乱血清型和鸡白痢血清型菌株进行了特征分析以评估耐药性。进行了抗菌药物敏感性测试、耐药基因和整合子检测、脉冲场凝胶电泳和质粒分析以对菌株进行特征分析。79%的菌株对至少一种测试抗菌药物耐药，而38%的菌株对六种或更多抗菌药物耐药。最常检测到对四环素（64%）、链霉素（57%）和卡那霉素（52%）的耐药性。总体而言，当观察到耐药性时，86.7%的情况下检测到相应的耐药基因。这项研究的结果表明，由于存在多重耐药性，抗菌药物耐药性是都柏林血清型和猪霍乱血清型菌株的一个主要问题。
