Piccione Guiseppe, Bertolucci Cristiano, Grasso Fortunata, Guidice Elisabetta
Department of Morphology, Biochemistry, Physiology and Animal Production, Faculty of Veterinary Medicine, University of Ferrara, Italy.
Vet Clin Pathol. 2007 Dec;36(4):358-60. doi: 10.1111/j.1939-165x.2007.tb00441.x.
The effect of storage temperature and time on blood gas and acid-base values has been investigated intensively in cattle and dogs; however, data are lacking in other species.
The aim of our study was to evaluate changes in gas composition and acid-base values in venous blood stored at different temperatures and for different times in 4 domestic species in Italy.
Blood samples from Comisana sheep (n = 10), Maltese goats (n = 10), Ragusana donkeys (n = 10), and Thoroughbred horses (n = 10) were analyzed after storage at 23 degrees C (room temperature) for 15 minutes (group I), 23 degrees C for 1 hour (group II), 37 degrees C for 8 hours (group III), and 4 degrees C for 24 hours (group IV). Results were analyzed using a 1-way repeated measures ANOVA.
In all species no statistically significant differences in pH values were present in samples stored at 4 degrees C for 24 hours. This also was true for PCO2 in all species except the horse. Except for HCO3- concentration in the horse, significant changes in PO2, HCO3- concentration, base excess, and the standard bicarbonate concentration were observed for all species in samples stored at 4 degrees C. In samples stored for only 1 hour at room temperature, significant changes in most analytes were detected.
The results of this study underline the need for rapid assessment of acid-base samples, because any delay, even for 1 hour, may affect the results.
关于储存温度和时间对牛和犬血气及酸碱值的影响已进行了深入研究；然而，其他物种的数据尚缺。
我们研究的目的是评估意大利4种家养动物的静脉血在不同温度下储存不同时间后气体成分和酸碱值的变化。
采集科米萨纳绵羊（n = 10）、马尔他山羊（n = 10）、拉古萨纳驴（n = 10）和纯种马（n = 10）的血样，分别在23℃（室温）下储存15分钟（I组）、23℃下储存1小时（II组）、37℃下储存8小时（III组）和4℃下储存24小时（IV组）后进行分析。结果采用单因素重复测量方差分析。
在所有物种中，4℃储存24小时的样本pH值无统计学显著差异。除马外，所有物种的PCO2也是如此。除马的HCO3 - 浓度外，4℃储存的样本中所有物种的PO2、HCO3 - 浓度、碱剩余和标准碳酸氢盐浓度均有显著变化。在室温下仅储存1小时的样本中，大多数分析物有显著变化。
本研究结果强调了对酸碱样本进行快速评估的必要性，因为任何延迟，即使仅1小时，都可能影响结果。
