Kennedy Sarah A, Constable Peter D, Sen Ismail, Couëtil Laurent
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
Am J Vet Res. 2012 Jul;73(7):979-87. doi: 10.2460/ajvr.73.7.979.
To determine effects of syringe type and storage conditions on blood gas and acid-base values for equine blood samples.
Blood samples obtained from 8 healthy horses.
Heparinized jugular venous blood was equilibrated via a tonometer at 37°C with 12% O(2) and 5% CO(2). Aliquots (3 mL) of tonometer-equilibrated blood were collected in random order by use of a glass syringe (GS), general-purpose polypropylene syringe (GPPS), or polypropylene syringe designed for blood gas analysis (PSBGA) and stored in ice water (0°C) or at room temperature (22°C) for 0, 5, 15, 30, 60, or 120 minutes. Blood pH was measured, and blood gas analysis was performed; data were analyzed by use of multivariable regression analysis.
Blood Po(2) remained constant for the reference method (GS stored at 0°C) but decreased linearly at a rate of 7.3 mm Hg/h when stored in a GS at 22°C. In contrast, Po(2) increased when blood was stored at 0°C in a GPPS and PSBGA or at 22°C in a GPPS; however, Po(2) did not change when blood was stored at 22°C in a PSBGA. Calculated values for plasma concentration of HCO(3) and total CO(2) concentration remained constant in the 3 syringe types when blood was stored at 22°C for 2 hours but increased when blood was stored in a GS or GPPS at 0°C.
Blood samples for blood gas and acid-base analysis should be collected into a GS and stored at 0°C or collected into a PSBGA and stored at room temperature.
确定注射器类型和储存条件对马血样血气及酸碱值的影响。
从8匹健康马采集的血样。
经肝素化处理的颈静脉血通过血气分析仪在37°C、含12%氧气和5%二氧化碳的条件下进行平衡。使用玻璃注射器（GS）、通用聚丙烯注射器（GPPS）或专为血气分析设计的聚丙烯注射器（PSBGA），以随机顺序收集3毫升经血气分析仪平衡的血液样本，并分别储存在冰水（0°C）或室温（22°C）下0、5、15、30、60或120分钟。测量血液pH值并进行血气分析；采用多变量回归分析对数据进行分析。
对于参考方法（储存在0°C的GS），血液氧分压（Po₂）保持恒定，但储存在22°C的GS中时，以7.3毫米汞柱/小时的速率呈线性下降。相比之下，当血液储存在0°C的GPPS和PSBGA中或22°C的GPPS中时，Po₂升高；然而，当血液储存在22°C的PSBGA中时，Po₂不变。当血液在22°C储存2小时时，三种注射器类型中计算得出的血浆碳酸氢根（HCO₃）浓度和总二氧化碳（CO₂）浓度值保持恒定，但当血液储存在0°C的GS或GPPS中时，这些值会升高。
用于血气和酸碱分析的血样应收集到GS中并储存在0°C，或收集到PSBGA中并储存在室温下。
