Matsui Ayu, Ashihara Hiroshi
Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo, Japan.
Plant Physiol Biochem. 2008 Jan;46(1):104-8. doi: 10.1016/j.plaphy.2007.10.008. Epub 2007 Oct 12.
Salvage of nicotinate riboside for NAD synthesis was investigated in mungbean seedlings. Nicotinate riboside kinase activity was detected in extracts from cotyledons. Exogenously supplied [carboxyl-(14)C]nicotinate riboside was readily converted into pyridine nucleotides in cotyledons of mungbean seedlings. This conversion was also found in embryonic axes, but the rate was lower than in cotyledons. These results suggest that, in addition to the seven-component pyridine nucleotide cycle (PNC VII), an eight-component cycle (PNC VIII) involving nicotinate riboside kinase operates in plants.
在绿豆幼苗中研究了烟酰胺核糖用于合成烟酰胺腺嘌呤二核苷酸(NAD)的补救作用。在子叶提取物中检测到烟酰胺核糖激酶活性。外源供应的[羧基-(14)C]烟酰胺核糖很容易在绿豆幼苗子叶中转化为吡啶核苷酸。在胚轴中也发现了这种转化,但速率低于子叶。这些结果表明,除了七组分吡啶核苷酸循环(PNC VII)外,植物中还存在一个涉及烟酰胺核糖激酶的八组分循环(PNC VIII)。