252Cf-plasma desorption and cesium-ion liquid secondary-ion mass spectrometric analysis of recombinant proteins.
作者信息
Tsarbopoulos A, Pramanik B N, Reichert P, Siegel M M, Nagabhushan T L, Trotta P P
机构信息
American Cyanamid Company, Lederle Laboratories, Pearl River, NY 10965.
出版信息
Rapid Commun Mass Spectrom. 1991 Feb;5(2):81-5. doi: 10.1002/rcm.1290050208.
PMID:1804408
Abstract
The 35 keV Cs+ liquid secondary-ion mass spectrometry (LSIMS) and 252Cf-plasma desorption (PD) mass spectra of recombinant proteins in the 10-25 kDa mass range are compared. Both techniques showed comparable mass accuracy and sensitivity, and in the case of LSIMS, remarkably short analysis time. Analysis by the PD/nitrocellulose method demonstrated slightly higher sensitivity and relatively lower dependence on the salt and buffer content of the protein sample.
摘要
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