Escolar Castellón J D, Roche Roche P A, Escolar Castellón A, Miñana Amada C
Department of Morphological Science, Faculty of Medicine, Zaragoza, Spain.
Histol Histopathol. 1991 Oct;6(4):535-47.
Two groups of rats with experimental alveolitis were exposed to cigarette smoke. After comparing the results, the possible muffling effect of the cigarette smoke related to interstitial lung disease was discussed. 180 rats were divided into 6 groups of 30 animals each: Group 1: untreated controls; Group 2: exposed to cigarette smoke for 2 months; Group 3: sensitized with bovine albumin (BA) and exposed to an atmosphere with this antigen for two months, to reproduce a type of extrinsic allergic alveolitis (EAA); Group 4: treated with a single daily dose of anti-lung serum for three days followed by two days without treatment, to reproduce a type of Goodpasture's syndrome; Group 5: exposed to cigarette smoke and to BA; Group 6: exposed to cigarette smoke and treated with anti-lung serum. The animals were sacrificed and their lungs were treated for: Bronchoalveolar lavage (BAL), percentage lymphocyte count, polymorphonuclear (PMN) and alveolar macrophages (AM); semiquantitative and morphometric histological study. The semiquantitative study determined the area of the studied lung incision, affected by granulomae, increased alveolar aerial spaces, thickened alveolar walls and haemosiderine lung area. The morphometric study, based on the linear integration method, evaluated: the distance between two alveolar walls, the amount of interstice per field; and the number of AM with haemosiderine per field was counted. From the results we point out that the treated animals had significantly higher lymphocyte and BAL PMN counts than the untreated ones; no significant differences were found between the singly and doubly treated animals. The animals exposed to cigarette smoke and treated with anti-lung serum were those that showed the least number of lymphocytes and PMN of all the treated animals. The semiquantitative variables studied were all increased in comparison to the control group, most of the increases being significant. The morphometric variables revealed significant differences with respect to the untreated group, except for the animals which were treated with anti-lung serum and cigarette smoke, which showed a minimum decrease in the alveolar size and a slight increase of the interstitial tissue. Only one morphometric variable showed a significant difference between the group treated with anti-lung serum and the one treated with anti-lung serum and cigarette smoke: the number of AM with haemosiderine in the lung.(ABSTRACT TRUNCATED AT 400 WORDS)
将两组患有实验性肺泡炎的大鼠暴露于香烟烟雾中。在比较结果后，讨论了香烟烟雾对间质性肺病可能的消音作用。180只大鼠被分为6组，每组30只动物：第1组：未治疗的对照组；第2组：暴露于香烟烟雾中2个月；第3组：用牛血清白蛋白（BA）致敏并在含有该抗原的环境中暴露2个月，以重现一种外源性过敏性肺泡炎（EAA）；第4组：每天单次给予抗肺血清治疗3天，随后2天不治疗，以重现一种Goodpasture综合征；第5组：暴露于香烟烟雾和BA中；第6组：暴露于香烟烟雾并接受抗肺血清治疗。处死动物后，对其肺部进行如下处理：支气管肺泡灌洗（BAL）、淋巴细胞计数百分比、多形核白细胞（PMN）和肺泡巨噬细胞（AM）；半定量和形态计量组织学研究。半定量研究确定了所研究的肺切片中受肉芽肿影响的面积、肺泡气腔增加、肺泡壁增厚和含铁血黄素肺面积。形态计量学研究基于线性积分法进行评估：两个肺泡壁之间的距离、每个视野中的间隙量；并计算每个视野中含铁血黄素的AM数量。从结果中我们指出，接受治疗的动物的淋巴细胞和BAL PMN计数显著高于未治疗的动物；单次和双重治疗的动物之间未发现显著差异。暴露于香烟烟雾并接受抗肺血清治疗的动物是所有接受治疗的动物中淋巴细胞和PMN数量最少的。与对照组相比，所研究的半定量变量均增加，大多数增加具有显著性。形态计量学变量显示与未治疗组有显著差异，但接受抗肺血清和香烟烟雾治疗的动物除外，这些动物的肺泡大小略有减小，间质组织略有增加。只有一个形态计量学变量在接受抗肺血清治疗的组和接受抗肺血清和香烟烟雾治疗的组之间显示出显著差异：肺中含铁血黄素的AM数量。（摘要截断于400字）
