Iriyama K, Tonouchi H, Azuma T, Suzuki H, Carpentier Y A
Second Department of Surgery, Mie University School of Medicine, Tsu, Japan.
Nutrition. 1991 Sep-Oct;7(5):355-7.
Acquisition of apolipoproteins C-II (apoCII) and C-III (apoCIII) is essential for the regulation of intravascular metabolism of fat particles (exoTG). This study was undertaken to investigate whether the capacity of high-density lipoprotein (HDL) for donating apoCII and apoCIII is influenced by the concentration of triglycerides (TGs) in plasma. A fat emulsion was infused into six male volunteers at a rate of 0.5 g TG.kg-1.h-1 (priming dose) for 30 min. For the following 160 min, fat was infused a fat emulsion was infused into the same subjects at a rate of 0.3 g.kg-1.h-1 for 160 min after the administration of the priming dose of fat emulsion for 30 min (experiment 2). The plasma TG concentrations and the amounts of apoCII and apoCIII in exo TG and HDL were monitored. The concentration of TG in the plasma stabilized at approximately 500 mg/dl in experiment 1, whereas it continued to increase to 815 +/- 42 mg/dl at 160 min after the start of the infusion of the fat emulsion in experiment 2. In experiment 1, the amount of both apoCII and apoCIII began to increase in exoTG and to decrease in HDL after the initiation of fat infusion. These changes in the distribution of apoC stabilized while the TG concentration remained at a plateau value. However, in experiment 2, the amount of apoC in exoTG did not increase further in response to the additional rise in plasma TG level. These results suggest that there is a relative lack of apoC that can be donated by HDL, depending on the quantitative balance between exoTG and HDL.(ABSTRACT TRUNCATED AT 250 WORDS)
获取载脂蛋白C-II（apoCII）和C-III（apoCIII）对于调节脂肪颗粒（外源性甘油三酯，exoTG）的血管内代谢至关重要。本研究旨在调查高密度脂蛋白（HDL）提供apoCII和apoCIII的能力是否受血浆中甘油三酯（TGs）浓度的影响。以0.5 g TG.kg-1.h-1的速率（起始剂量）向6名男性志愿者输注脂肪乳剂30分钟。在接下来的160分钟内，在给予起始剂量的脂肪乳剂30分钟后，以0.3 g.kg-1.h-1的速率向同一受试者输注脂肪乳剂160分钟（实验2）。监测血浆TG浓度以及exo TG和HDL中apoCII和apoCIII的量。实验1中血浆TG浓度稳定在约500 mg/dl，而在实验2中脂肪乳剂输注开始后160分钟时，其持续升高至815±42 mg/dl。在实验1中，脂肪输注开始后，exoTG中apoCII和apoCIII的量均开始增加，而HDL中的量则开始减少。apoC分布的这些变化在TG浓度保持在平台值时稳定下来。然而，在实验2中，exoTG中apoC的量并未因血浆TG水平的进一步升高而进一步增加。这些结果表明，根据exoTG和HDL之间的定量平衡，HDL可提供的apoC相对缺乏。（摘要截断于250字）
