Yamada H, Hirano M, Kiyohara H
Oriental Medicine Research Center of the Kitasato Institute, Tokyo, Japan.
Carbohydr Res. 1991 Oct 14;219:173-92. doi: 10.1016/0008-6215(91)89050-p.
Methylation analysis of a pectic polysaccharide (Bupleuran 2IIc) with anti-ulcer activity, isolated from the roots of Bupleurum falcatum L., revealed (1----4)-linked alpha-GalA together with small proportions of 2,4- and 3,4-linked GalA, and variously linked neutral sugars. Digestion of Bupleuran 2IIc with endo-alpha-(1----4)-polygalacturonase gave mainly galacturono-oligosaccharides (PG-4) and small proportions of enzyme-resistant regions (PG-1-3). PG-1 contained the sequence----4)GalA- (1----2)-Rha-(1----4)-GalA-(1----, and partial acid hydrolysis gave GalA-(1----4)-Rha, GlcA-(1----4)-Rha, and several di- and oligosaccharides consisting variously of Xyl, Glc, Gal, and Man. PG-2 and PG-3 each contained Rha, Fuc, Ara, Xyl, Man, Gal, Glc, GalA, GlcA, 2-Me-Fuc, 2-Me-Xyl, apiose (Api), aceric acid (AceA), and 3-deoxy-D-manno-2-octulosonic acid (Kdo). PG-4 contained (1----4)-linked alpha-galacturono-di-to-penta- saccharides and GalA. The galacturono-tetra- and -penta-saccharides had one and three methyl-esterified GalA units, respectively, and some of the galacturono-oligosaccharides contained 2,4- or 2,3-linked GalA.
从柴胡（Bupleurum falcatum L.）根部分离得到的具有抗溃疡活性的果胶多糖（柴胡2IIc）的甲基化分析表明，其含有（1→4）连接的α-半乳糖醛酸（α-GalA），以及少量2,4-和3,4-连接的半乳糖醛酸（GalA）和各种连接方式的中性糖。用内切α-（1→4）-聚半乳糖醛酸酶消化柴胡2IIc主要得到半乳糖醛酸寡糖（PG-4）和少量酶抗性区域（PG-1-3）。PG-1含有序列→4）GalA-（1→2）-Rha-（1→4）-GalA-（1→，部分酸水解得到GalA-（1→4）-Rha、GlcA-（1→4）-Rha，以及几种由木糖（Xyl）、葡萄糖（Glc）、半乳糖（Gal）和甘露糖（Man）组成的二糖和寡糖。PG-2和PG-3各自含有鼠李糖（Rha）、岩藻糖（Fuc）、阿拉伯糖（Ara）、木糖（Xyl）、甘露糖（Man）、半乳糖（Gal）、葡萄糖（Glc）、半乳糖醛酸（GalA）、葡萄糖醛酸（GlcA）、2-甲基岩藻糖（2-Me-Fuc）、2-甲基木糖（2-Me-Xyl）、芹糖（Api）、乙酰基酸（AceA）和3-脱氧-D-甘露-2-辛酮糖酸（Kdo）。PG-4含有（1→4）连接的α-半乳糖醛酸二糖至五糖和半乳糖醛酸。半乳糖醛酸四糖和五糖分别含有一个和三个甲基酯化的半乳糖醛酸单元，一些半乳糖醛酸寡糖含有2,4-或2,3-连接的半乳糖醛酸。
