Yamada H, Ra K S, Kiyohara H, Cyong J C, Otsuka Y
Oriental Medicine Research Center of the Kitasato Institute, Tokyo, Japan.
Carbohydr Res. 1989 Jun 15;189:209-26. doi: 10.1016/0008-6215(89)84098-4.
An anti-complementary pectic polysaccharide (BR-2-IIb), isolated from the roots of Bupleurum falcatum L., has an average molecular weight of 36,000 (gel filtration), and was subjected to methylation analysis before and after carboxyl-reduction, digestion with endo-polygalacturonase, base-catalysed beta-elimination, and partial acid hydrolysis. BR-2-IIb consisted mainly of galacturonic acid, arabinose, rhamnose, and galactose in the molar ratios 13.0:2.1:1.4:1.0 and contained a large enzyme-sensitive polygalacturonan region. The enzyme-resistant region (PG-1) was rich in neutral sugars and contained a backbone of 4-linked GalA and 2-linked Rha to which a highly branched arabinogalactan was attached to position 4 of some 2-linked Rha units. Partial acid hydrolysis of BR-2-IIb gave Ara-(1----3)-Ara, Ara-(1----4)-Arap, Ara-(1----5)-Araf, Ara-(1----6)-Gal, Gal-(1----4)-Gal, GalA-(1----2)-Rha, GalA-(1----4)-Rha, GalA----Rha----Rha, Gal----Rha----Rha, and GlA-(1----6)-Gal in addition to (1----4)linked oligogalacturonides. The anticomplementary activity of BR-2-IIb was enhanced by de-esterification, but carboxyl-reduction decreased the activity.
从柴胡根部分离得到的一种抗补体果胶多糖（BR-2-IIb），通过凝胶过滤法测得其平均分子量为36,000，在进行羧基还原、用内切聚半乳糖醛酸酶消化、碱催化β-消除和部分酸水解前后分别进行了甲基化分析。BR-2-IIb主要由半乳糖醛酸、阿拉伯糖、鼠李糖和半乳糖组成，摩尔比为13.0:2.1:1.4:1.0，并且含有一个大的对酶敏感的聚半乳糖醛酸区域。抗酶解区域（PG-1）富含中性糖，包含由4-连接的半乳糖醛酸和2-连接的鼠李糖组成的主链，在一些2-连接的鼠李糖单元的4位连接有高度分支化的阿拉伯半乳聚糖。BR-2-IIb的部分酸水解产物除了（1→4）连接的低聚半乳糖醛酸外，还有Ara-(1→3)-Ara、Ara-(1→4)-Arap、Ara-(1→5)-Araf、Ara-(1→6)-Gal（原文有误，推测为Ara-(1→6)-GalA）、Gal-(1→4)-Gal、GalA-(1→2)-Rha、GalA-(1→4)-Rha、GalA→Rha→Rha、Gal→Rha→Rha以及GlA-(1→6)-Gal（原文有误，推测为GalA-(1→6)-Gal）。BR-2-IIb的抗补体活性通过去酯化作用增强，但羧基还原会降低其活性。 （括号内为推测修正原文错误后的内容，使译文更符合逻辑）
