Ma Lu, Wu Sheng-Mei, Huang Jing, Ding Yi, Pang Dai-Wen, Li Lijia
Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
Chromosoma. 2008 Apr;117(2):181-7. doi: 10.1007/s00412-007-0136-2. Epub 2007 Nov 29.
Semiconductor nanocrystals, also called quantum dots (QDs), are novel inorganic fluorophores which are brighter and more photostable than organic fluorophores. In the present study, highly dispersive QD-labeled oligonucleotide (TAG)(8) (QD-deoxyribonucleic acid [DNA]) conjugates were constructed via the metal-thiol bond, which can be used as fluorescence in situ hybridization (FISH) probes. FISH analysis of maize metaphase chromosomes using the QD-DNA probes showed that the probes could penetrate maize chromosomes and nuclei and solely hybridized to complementary target DNAs. Compared with the conventional organic dyes such as Cy3 and fluorescein isothiocyanate, this class of luminescent labels bound with oligonucleotides is brighter and more stable against photobleaching on the chromosomes after FISH. These results suggest that QD fluorophores may be a more stable and useful fluorescent label for FISH applications in plant chromosome mapping considering their size-tunable luminescence spectra.
半导体纳米晶体，也称为量子点（QDs），是新型无机荧光团，比有机荧光团更亮且光稳定性更高。在本研究中，通过金属 - 硫醇键构建了高度分散的量子点标记寡核苷酸（TAG）(8)（量子点 - 脱氧核糖核酸[DNA]）缀合物，其可用作荧光原位杂交（FISH）探针。使用量子点 - DNA探针对玉米中期染色体进行FISH分析表明，探针可穿透玉米染色体和细胞核，并仅与互补靶DNA杂交。与传统有机染料如Cy3和异硫氰酸荧光素相比，这类与寡核苷酸结合的发光标记在FISH后在染色体上更亮且抗光漂白更稳定。这些结果表明，考虑到量子点荧光团具有尺寸可调的发光光谱，它们可能是用于植物染色体图谱绘制中FISH应用的更稳定且有用的荧光标记。
