Xiao Yan, Barker Peter E
GeoCenters, Inc. and DNA Technologies Group, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA .
Nucleic Acids Res. 2004 Feb 11;32(3):e28. doi: 10.1093/nar/gnh024.
To improve signal stability and quantitation, an optically stable, novel class of fluorophore for hybridization analysis of human metaphase chromosomes is demonstrated. Detection of hybridization sites in situ was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide [(CdSe)ZnS]. Fluorescence of nanocrystal fluorophores was significantly brighter and more photostable than organic fluorophores Texas Red and fluorescein. Thus, semiconductor nanocrystal fluorophores offer a more stable and quantitative mode of fluorescence in situ hybridization (FISH) for research and clinical applications.
为提高信号稳定性和定量分析能力，展示了一种光学稳定的新型荧光团，用于人类中期染色体的杂交分析。原位杂交位点的检测基于与链霉亲和素相连的硒化镉[(CdSe)ZnS]无机晶体的荧光。纳米晶体荧光团的荧光比有机荧光团德克萨斯红和荧光素明显更亮且光稳定性更好。因此，半导体纳米晶体荧光团为研究和临床应用提供了一种更稳定、定量的荧光原位杂交（FISH）模式。
