Wang Yan-Lin, Wang Cheng-Yao, Zhang Ben-Jing, Zhang Zong-Ze
Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Donghu Road 169, Wuhan 430071, China.
Mol Biol Rep. 2009 Feb;36(2):365-70. doi: 10.1007/s11033-007-9188-x. Epub 2007 Nov 30.
Shenfu injection (the major components of which are ginsenosides compound, extract of Panax ginseng shown to have antioxidant properties) is a well-known important Chinese traditional medicine used for the treatment of various diseases especial for cardiac diseases. The precise mechanism of the biological actions of this plant is not fully understood, in order to elucidate the protection of cardiomyocytes. The aim of the present study was to investigate the effect of Shenfu injection on hypoxia/reoxygenation (H/R)-induced apoptosis and the expression of bcl-2 and caspase-3 in cultured neonatal rat cardiomyocytes in vitro. Ventricular myocytes were isolated from neonatal rat hearts and were exposed to 4 h of hypoxia followed by 16 h of reoxygenation. The results indicated that treatment with different doses of Shenfu injection protected cardiacmyocyte cultures from hypoxia/reoxygenation-induced apoptosis. Caspase-3 activation was decreased in hypoxic/reoxygenationed cardiomyocytes co-treated with Shenfu injection when compared to hypoxia/reoxygenation alone treated cultures. Expression of the Bcl-2 proteins was increased in Shenfu injection-treated cardiomyocytes subjected to hypoxia/reoxygenation. In conclusion, ginsenosides compound has obviously protective effects on cardiacmyocytes against apoptosis induced by hypoxia/reoxygenation injury, whose mechanisms probably involve the inhibition of down-regulation of Bcl-2 protein levels and sequential activation of caspase-3.
参附注射液（其主要成分是复方人参皂苷，人参提取物具有抗氧化特性）是一种著名的重要中药，用于治疗各种疾病，尤其适用于心脏病。这种植物生物作用的确切机制尚未完全了解，为了阐明对心肌细胞的保护作用。本研究的目的是探讨参附注射液对体外培养的新生大鼠心肌细胞缺氧/复氧（H/R）诱导的细胞凋亡以及bcl-2和caspase-3表达的影响。从新生大鼠心脏分离心室肌细胞，使其暴露于4小时缺氧，随后再进行16小时复氧。结果表明，不同剂量的参附注射液处理可保护心肌细胞培养物免受缺氧/复氧诱导的细胞凋亡。与单独进行缺氧/复氧处理的培养物相比，在与参附注射液共同处理的缺氧/复氧心肌细胞中，caspase-3的激活减少。在经历缺氧/复氧的参附注射液处理的心肌细胞中，Bcl-2蛋白的表达增加。总之，人参皂苷复方对缺氧/复氧损伤诱导的心肌细胞凋亡具有明显的保护作用，其机制可能涉及抑制Bcl-2蛋白水平的下调和caspase-3的顺序激活。
