Marton Szilvia, Eszterbauer Edit
Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary.
Folia Parasitol (Praha). 2011 Jun;58(2):157-63. doi: 10.14411/fp.2011.015.
Echinactinomyxon-type actinospores were found in a mixed-species oligochaete culture originating from the Temperate Water Fish Hatchery near Budapest, Hungary. On the basis of DNA sequence analysis, the actinospores were identified as Myxobolus pavlovskii (Akhmerov, 1954), the 18S rDNA sequence from myxospores of which is available in GenBank. Silver carp Hypophthalmichthys molitrix (Valenciennes) fry specimens were successfully infected by cohabitation with the echinactinomyxon-releasing oligochaetes, which confirmed the molecular data congruence. The echinactinomyxons and the myxospores that developed in the gills of exposed fish fry were analysed morphologically and on DNA basis. The infected gill tissue was examined histologically. As typical characters of M. pavlovskii, numerous small plasmodia were observed in the epithelia of gill lamellae. Plasmodia contained thousands of myxospores with polar capsules unequal in size and witl; large intercapsular processes. The 18S rDNA sequence from actinospores and those from myxospores originating from the experimentally infected fish were identical. The oligochaete species releasing actinospores was morphologically determined as Limnodrilus sp. This is the first record of an echinactinomyxon as an alternate stage within the genus Myxobolus.
在源自匈牙利布达佩斯附近温带水产养殖场的混合种类寡毛纲动物培养物中发现了棘黏孢子虫型放射孢子。基于DNA序列分析，这些放射孢子被鉴定为帕氏黏体虫（Akhmerov，1954年），其黏孢子的18S rDNA序列可在GenBank中获取。通过与释放棘黏孢子虫的寡毛纲动物同居，成功感染了鲢鱼Hypophthalmichthys molitrix（瓦朗谢讷）鱼苗样本，这证实了分子数据的一致性。对暴露鱼苗鳃中发育的棘黏孢子虫和黏孢子进行了形态学和DNA分析。对感染的鳃组织进行了组织学检查。作为帕氏黏体虫的典型特征，在鳃小片上皮中观察到大量小的原质团。原质团包含数千个黏孢子，其极囊大小不等且有大的囊间突。来自放射孢子和实验感染鱼的黏孢子的18S rDNA序列相同。释放放射孢子的寡毛纲动物种类在形态上被确定为水蚯蚓属物种。这是棘黏孢子虫作为黏体虫属内交替阶段的首次记录。
