Fleischhacker M, Radecke C, Schulz B, Ruhnke M
Department of Internal Medicine, Div. Oncology and Hematology, Charité Universitätsmedizin Berlin, Charité Campus Mitte, Charitéplatz 1, 10117 Berlin, Germany.
Eur J Clin Microbiol Infect Dis. 2008 Feb;27(2):127-31. doi: 10.1007/s10096-007-0411-4. Epub 2007 Dec 4.
To analyze the effects of a high concentration of three antifungal substances, the echinocandins anidulafungin, caspofungin, and micafungin, on the growth of Candida spp.
The growth of 127 C. dubliniensis isolates and 103 C. albicans isolates cultured in medium containing anidulafungin, caspofungin, or micafungin was analyzed using a broth microdilution test according to the guidelines of the CLSI M27-A2 [NCCLS (1997), Wayne, PA]. The final concentrations of all three echinocandins ranged from 0.125 to 64 microg/L.
The different effects of these three antifungal substances on C. albicans cells in comparison to C. dubliniensis cells were quite distinct. When both Candida species were grown in the presence of anidulafungin only a trailing effect was observed. Micafungin induced an Eagle effect in C. dubliniensis only (63%), while caspofungin induced this effect in the majority of C. dubliniensis isolates (90%) and in only a few C. albicans isolates (14%).
Based on our observations, anidulafungin has effects that are different from the ones produced by micafungin and caspofungin. Whether this different response to high concentrations of echinocandins is based on genetic or phenotypic differences between C. albicans and C. dubliniensis has to be determined in future experiments.
分析三种高浓度抗真菌物质（棘白菌素类的阿尼芬净、卡泊芬净和米卡芬净）对念珠菌属生长的影响。
根据CLSI M27 - A2 [NCCLS（1997年），宾夕法尼亚州韦恩市]指南，采用肉汤微量稀释法分析在含有阿尼芬净、卡泊芬净或米卡芬净的培养基中培养的127株都柏林念珠菌分离株和103株白色念珠菌分离株的生长情况。所有三种棘白菌素的终浓度范围为0.125至64μg/L。
与都柏林念珠菌细胞相比，这三种抗真菌物质对白色念珠菌细胞的不同作用相当明显。当两种念珠菌在仅存在阿尼芬净的情况下生长时，仅观察到拖尾效应。米卡芬净仅在都柏林念珠菌中诱导伊格尔效应（63%），而卡泊芬净在大多数都柏林念珠菌分离株（90%）和少数白色念珠菌分离株（14%）中诱导这种效应。
根据我们的观察，阿尼芬净的作用与米卡芬净和卡泊芬净不同。白色念珠菌和都柏林念珠菌对高浓度棘白菌素的这种不同反应是基于基因差异还是表型差异，必须在未来的实验中确定。
