Cao Hui, Xu Shi-Ying
School of Food Science and Technology, Southern Yangtze University, No. 1800 Lihu Road, Wuxi, Jiangsu, China.
J Mater Sci Mater Med. 2008 Feb;19(2):567-75. doi: 10.1007/s10856-007-3281-5. Epub 2007 Dec 6.
Three-dimensional biodegradable porous type II collagen scaffolds are interesting materials for cartilage tissue engineering. This study reports the preparation of porous type II collagen-chondroitin sulfate (CS) scaffold using variable concentrations of 1-ethyl-3(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The physico-chemical properties and ultrastructural morphology of the collagen scaffolds were determined. Then, isolated chondrocytes were cultured in porous type II collagen scaffolds either in the presence and/or absence of covalently attached CS up to 14 days. Cell proliferation, the total amount of proteoglycans and type II collagen retained in the scaffold and chondrocytes morphology were evaluated. The results suggest that EDC-crosslinking improves the mechanical stability of collagen-CS scaffolds with increasing EDC concentration. Cell proliferation and the total amount of proteoglycans and type II collagen retained in the scaffolds were higher in type II collagen-CS scaffolds. Histological analysis showed the formation of a denser cartilaginous layer at the scaffold periphery. Scanning electron microscopy (SEM) revealed chondrocytes distributed the porous surface of both scaffolds maintained their spherical morphology. The results of the present study also indicate that type II collagen-CS scaffolds have potential for use in tissue engineering.
三维可生物降解多孔II型胶原支架是软骨组织工程中令人感兴趣的材料。本研究报道了使用不同浓度的1-乙基-3-(3-二甲基氨基丙基)碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)制备多孔II型胶原-硫酸软骨素(CS)支架。测定了胶原支架的物理化学性质和超微结构形态。然后，将分离的软骨细胞在多孔II型胶原支架中培养14天，培养过程中存在和/或不存在共价连接的CS。评估了细胞增殖、支架中保留的蛋白聚糖总量和II型胶原以及软骨细胞形态。结果表明，随着EDC浓度的增加，EDC交联提高了胶原-CS支架的机械稳定性。II型胶原-CS支架中的细胞增殖以及支架中保留的蛋白聚糖总量和II型胶原更高。组织学分析显示在支架周边形成了更致密的软骨层。扫描电子显微镜(SEM)显示，分布在两种支架多孔表面的软骨细胞保持其球形形态。本研究结果还表明，II型胶原-CS支架具有用于组织工程的潜力。
