寡核苷酸-肽缀合物的制备。
Preparation of oligonucleotide-peptide conjugates.
作者信息
Tung C H, Rudolph M J, Stein S
机构信息
Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey 08854.
出版信息
Bioconjug Chem. 1991 Nov-Dec;2(6):464-5. doi: 10.1021/bc00012a015.
PMID:1805945
Abstract
A procedure for preparing oligonucleotide-peptide conjugates is presented. It is based on appending a maleimide group to the oligonucleotide for selective coupling to the thiol side chain of a cysteine residue in the peptide. A convenient chromatographic purification procedure, based on Fmoc-on/Fmoc-off, is described.
摘要
本文介绍了一种制备寡核苷酸-肽缀合物的方法。该方法基于在寡核苷酸上连接一个马来酰亚胺基团，以便与肽中半胱氨酸残基的硫醇侧链进行选择性偶联。还描述了一种基于Fmoc保护基的引入与去除的简便色谱纯化方法。
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